Erastin2
Based on 6 publication(s) in Google Scholar
Erastin2, an Erastin (HY-15763) analog, is a ferroptosis inducer and a potent, selective inhibitor of the system xc(-) cystine/glutamate transporter
For research use only. We do not sell to patients.
- Purity: 98.76%
- CAS No.: 1695533-44-8
- Formula: C36H35ClN4O4
- Molecular Weight:623.14
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Storage:
4°C, protect from light
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Ferroptosis Compound Library
HY-L051
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Endoplasmic Reticulum Stress Compound Library
HY-L054
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Highly Selective Activators Library
HY-L159
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Cell Death Library
HY-L162
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Bioactive Compound Library Max
HY-L181
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MCE Bioactive Compound Library
HY-L001V
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Bioactive Compound Library
HY-L001
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High-Throughput Bioactive Compound Library
HY-L205
Publications Citing Use of MedChemExpress (MCE) Erastin2
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Customer Validation & Images
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Cell Proliferation/Viability Assay
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Cell Proliferation/Viability Assay
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Microbiological Assay
Biological Activity
Erastin2 (2 μM, 48 h) induced ferroptosis correlates with acute amino acid deprivationinduced proliferative arrest in a manner that can be independent of mTOR inhibition and GCN2/ATF4 pathway activation in both U-2 OSN and HT-1080N cells in response to erastin2[1]. Erastin2 (1 μM, 48 h) -induced HT-1080 TP53 KO fibrosarcoma cell death are sensitive regardless of nutlin-3 pretreatment [4]. p53 sabilization suppresses Erastin2 (1 μM, 48 h) -induced ferroptosis in response to system xc(-) inhibition in HT-1080 TP53 KO fibrosarcoma cells[4].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:HT-1080N fibrosarcoma cells
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Concentration:1 or 2 μM
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Incubation Time:48 h
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Result:Consistently initiated cell death 8 h earlier than Camptothecin (HY-16560), such that Camptothecin (HY-16560) may not have time to act before the induction of ferroptosis has already committed cells to death via this pathway in HT-1080N fibrosarcoma cells. The six most potent compounds (Fer-1, SKI II, phenothiazine, JNJ-26854165, AZD3463, and bazedoxifene) all suppressed Erastin2 and ML162-induced ferroptosis with sub-150 nM potency in HT-1080N fibrosarcoma cells.
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Cell Line:U-2 OSN and HT-1080N cells
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Concentration:2 μM
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Incubation Time:48 h
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Result:Deprivation of glutamine, lysine, valine, methionine, and arginine, all suppressed ferroptosis in both U-2 OSN and HT-1080N cells in response to erastin2.
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Cell Line:Human HT-1080 TP53 KO fibrosarcoma cells
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Concentration:1 μM, pretreated for 48 h with or without Nutlin-3 (HY-50696)
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Incubation Time:48 h
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Result:Control cells pretreated with nutlin-3 were less sensitive to erastin2-induced cell death, by contrast, TP53 KO cells were equally sensitive to erastin2 regardless of nutlin-3 pretreatment.
Chemical Information
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CAS No. 1695533-44-8
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Appearance Solid
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Molecular Weight 623.14
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Formula C36H35ClN4O4
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Color White to off-white
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SMILES
O=C1C2=C(N=C(CN3CCN(CC3)C(COC4=CC=C(C=C4)Cl)=O)N1C5=C(OC(C)C)C=CC(C6=CC=CC=C6)=C5)C=CC=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 2 years; -20°C, 1 year (protect from light)
Publications (6)
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Journal Impact Factor
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Most Recent
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Nature
2026 Jan;649(8096):477-486. PMID: 41193799
Erastin2 purchased from MedChemExpress. Usage Cited in: Nature. 2026 Jan;649(8096):477-486. [Abstract]
Cell viability of B16-F0 and LN metastatic lines treated with increasing concentrations of Erastin2 or 0.5 μM for 24 h.
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Adv Sci (Weinh)
2026 Feb 17:e23198. PMID: 41704007
Erastin2 purchased from MedChemExpress. Usage Cited in: Adv Sci (Weinh). 2026 Feb 17:e23198. [Abstract]
Cancer cells were isolated from the resected primary tumor in 4T1‐Balb/c and M231‐NSG models and incubated with dose‐range RSL3 and Erastin2 for 48 h. Cell viability was assessed using an absorbance‐based assay (MTT), and results are expressed as a ratio to the untreated condition (n = 6–8 mice per group).
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Antimicrob Agents Chemother
Synergistic activity of RSL3 and Pyrimethamine to inhibit the proliferation of Plasmodium falciparum. [Abstract]2025 Aug 18:e0047125. PMID: 40823867
Erastin2 purchased from MedChemExpress. Usage Cited in: Antimicrob Agents Chemother. 2025 Aug 18:e0047125. [Abstract]
Erastin2 (0.125-2 μM) inhibited the proliferation of P. falciparum as single agents, yet none of them exhibited synergy with Pyrimethamine.
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bioRxiv
2026 Mar 30:2026.03.28.714855. PMID: 41959534 -
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Solvent & Solubility
DMSO : 100 mg/mL (160.48 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (279 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
[1]. Conlon M, et al. A compendium of kinetic modulatory profiles identifies ferroptosis regulators[J]. Nat Chem Biol. 2021 Jun;17(6):665-674. [Content Brief]
[2]. Cao JY, et al. A Genome-wide Haploid Genetic Screen Identifies Regulators of Glutathione Abundance and Ferroptosis Sensitivity. Cell Rep. 2019 Feb 5;26(6):1544-1556.e8. [Content Brief]
[3]. Dixon SJ, et al. Pharmacological inhibition of cystine-glutamate exchange induces endoplasmic reticulum stress and ferroptosis. Elife. 2014 May 20;3:e02523. [Content Brief]
[4]. Tarangelo A, et al. p53 Suppresses Metabolic Stress-Induced Ferroptosis in Cancer Cells. Cell Rep. 2018 Jan 16;22(3):569-575. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year (protect from light). When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.6048 mL | 8.0239 mL | 16.0478 mL | 40.1194 mL |
| 5 mM | 0.3210 mL | 1.6048 mL | 3.2096 mL | 8.0239 mL | |
| 10 mM | 0.1605 mL | 0.8024 mL | 1.6048 mL | 4.0119 mL | |
| 15 mM | 0.1070 mL | 0.5349 mL | 1.0699 mL | 2.6746 mL | |
| 20 mM | 0.0802 mL | 0.4012 mL | 0.8024 mL | 2.0060 mL | |
| 25 mM | 0.0642 mL | 0.3210 mL | 0.6419 mL | 1.6048 mL | |
| 30 mM | 0.0535 mL | 0.2675 mL | 0.5349 mL | 1.3373 mL | |
| 40 mM | 0.0401 mL | 0.2006 mL | 0.4012 mL | 1.0030 mL | |
| 50 mM | 0.0321 mL | 0.1605 mL | 0.3210 mL | 0.8024 mL | |
| 60 mM | 0.0267 mL | 0.1337 mL | 0.2675 mL | 0.6687 mL | |
| 80 mM | 0.0201 mL | 0.1003 mL | 0.2006 mL | 0.5015 mL | |
| 100 mM | 0.0160 mL | 0.0802 mL | 0.1605 mL | 0.4012 mL |
Erastin2 Related Classifications
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