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Cat. No.: HY-P991443

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IPH5301

IPH5301 is a humanized monoclonal antibody targeting human CD73. IPH5301 potently blocks the ectonucleotidase activity of both membrane-bound and soluble CD73, preventing the degradation of AMP to immunosuppressive adenosine, thereby relieving adenosine-mediated inhibition of T cell proliferation and activation. IPH5301 shows synergistic antitumor effects with the anti-CD39 antibody IPH5201 (HY-P991444). IPH5301 can be used in research on breast cancer, melanoma, acute myeloid leukemia, and fibrosarcoma.

For research use only. We do not sell to patients.

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (271 KB)

IPH5301 Chemical Structure

Price

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Use of MCE Products

One-stop solution for XDC (ADC/PDC/RDC/ApDC/FDC/ISAC/VDC/SMDC)

Biological Activity
- Description
- Isotype
- Recommend Isotype Controls
- Species Reactivity
- IC₅₀ & Target
- In Vitro
- In Vivo
- Clinical Trial
- Gene ID
- Accession
- Conjugated
- Reconsititution
- Application
Chemical Information
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
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Biological Activity

Description

Isotype

Human IgG1 kappa

Recommend Isotype Controls

Human IgG1 kappa, Isotype Control

Species Reactivity

Human

IC₅₀ & Target

NT5E/CD73

In Vitro

Adaptive ImmunityAnti-cancerInnate ImmunityOthers

Adaptive Immunity

IPH5301 dose-dependently reverses the inhibition of T cell proliferation induced by exogenous AMP or ATP (HY-B2176) (when used in combination with an anti-CD39 antibody) in peripheral blood CD4+ and CD8+ T cells from healthy or breast cancer samples activated with anti-CD3/CD28 (HY-K0353)^[1].

Anti-cancer

IPH5301 (1-2 h) blocks the hydrolysis of AMP (HY-A0181) to adenosine by both membrane-bound and soluble CD73 in A375 human melanoma cells and MDA-MB-231 breast cancer cells (and their cell culture supernatants) in a dose-dependent and potent manner without inducing receptor internalization^[1].

Innate Immunity

Adaptive Immunity

IPH5301 (5-7 days) reverses ATP-mediated immunosuppression and restores T cell proliferation in a co-culture system of human monocyte-derived dendritic cells (MoDCs) and allogeneic CD4+ T cells^[1].

Others

IPH5301 binds the CD73 dimer at a 1:1 stoichiometric ratio, trapping CD73 in an intermediate conformation incompatible with AMP hydrolysis and blocking CD73 enzymatic activity^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

Anti-cancer

IPH5301 shows synergistic antitumor activity with IPH5201 in mouse melanoma models and fibrosarcoma models^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Related Protocols :

Clinical Trial

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991			2011-05	PHASE1

Gene ID

4907 [NCBI]

Accession

P21589

Conjugated

Unconjugated

Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.

Application

ELISA, FACS, Functional assay

Chemical Information

SMILES

[IPH5301]
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (271 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Inhibitory Antibodies User Guide (603 KB)

References

[1]. Perrot I, et al. Blocking Antibodies Targeting the CD39/CD73 Immunosuppressive Pathway Unleash Immune Responses in Combination Cancer Therapies. Cell reports. 2019 May 21;27(8):2411-2425.e9.

[2]. Wang L, et al. Enhanced CD33 CAR-NK cells secreting anti-CD73scFv overcome adenosine-mediated immunosuppression and improve anti-AML efficacy. Journal for immunotherapy of cancer. 2026 Sep 01;14(9):e014825.

[3]. Chambers AM, et al. Immunometabolic Dysfunction of Natural Killer Cells Mediated by the Hypoxia-CD73 Axis in Solid Tumors. Frontiers in molecular biosciences. 2019;6:60.

Calculators

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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Keywords

MOQ

Minimum order quantity

100 mg

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