F-L319

C57BL/6 (female, 6−8 weeks old)^[2]

2 μg mLuc per mouse

i.v.; single dose

Barely exhibited any mRNA expression in vivo, with negligible bioluminescence signals at all tested time points.
Showed nearly no detectable mRNA expression in major organs (spleen, liver, heart, lung, kidney) at 24 h post-injection.\nExhibited significantly higher bioluminescence signals than L319-LNP at 4 h, 12 h, and 24 h post-injection; signals were comparable to Hybrid-LNP2 at all time points.
Showed targeted mRNA expression primarily in the spleen at 24 h post-injection, with much weaker expression in the liver and nearly no expression in other major organs.\nProduced stronger bioluminescence signals than Hybrid-LNP1 at 4 h post-injection, though signals declined more rapidly over time; signals were significantly higher than L319-LNP at all tested time points.
Showed targeted mRNA expression primarily in the spleen at 24 h post-injection, with much weaker expression in the liver and nearly no expression in other major organs.