FITC-D-Glucose
Based on 1 Customer Validation
FITC-D-Glucose is a fluorescently labeled glucose derivative and an orally effective bacterial metabolic activity detection probe. FITC-D-Glucose is applicable to the research of cholera.
For research use only. We do not sell to patients.
- Formula: C27H24N2O10S
- Molecular Weight:568.55
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Storage:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Biological Activity
FITC-D-Glucose (1 μL in 10 mL Met-free MM medium; 30 min at 37 °C) labeling reveals that flhB mutant V. cholerae has 1.7-fold higher in vitro metabolic activity than wild-type, while flaA mutant has 0.6-fold lower in vitro metabolic activity than wild-type[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:CD-1 (5-week-old)[1]
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Dosage:0.1 mM
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Administration:p.o.; single 100 μL dose
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Result:Labeled metabolically active V.
cholerae ΔflhB mutants, resulting in a 7-fold higher high metabolically active cell rate than that of wild-type.
Labeled metabolically active V.
cholerae ΔflaA mutants, resulting in a 0.2-fold high metabolically active cell rate relative to wild-type.
Chemical Information
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Appearance Solid
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Molecular Weight 568.55
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Formula C27H24N2O10S
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SMILES
O=C(C1=C2C=CC(NC(N[C@@H]([C@H](O)[C@@H](O)C(CO)O3)[C@H]3O)=S)=C1)OC42C5=C(C=C(O)C=C5)OC6=CC(O)=CC=C64
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : 4 mg/mL (7.04 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7589 mL | 8.7943 mL | 17.5886 mL | 43.9715 mL |
| 5 mM | 0.3518 mL | 1.7589 mL | 3.5177 mL | 8.7943 mL |