Flunamine
Flunamine is a broad-spectrum monoamine transporter inhibitor that inhibits the uptake of dopamine (DA), 5-hydroxytryptamine (5-HT), and noradrenaline (NA) in rat brain synaptosomes. The IC50 values of Flunamine for DA and 5-HT uptake in rat striatal synaptosomes are 0.26 μM and 0.17 μM, respectively, and the IC50 values for 5-HT and NA uptake in hypothalamic synaptosomes are 0.24 μM and 0.092 μM, respectively. Flunamine acts through broad-spectrum inhibition of dopaminergic, serotonergic, and noradrenergic monoamine reuptake and is used in research related to depression and Parkinson's disease.
For research use only. We do not sell to patients.
- CAS No.: 50366-32-0
- Formula: C15H15F2NO
- Molecular Weight:263.29
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[2]|
DAT 0.26 μM (IC50) |
SERT 0.17 μM (IC50) |
SERT 0.24 μM (IC50) |
NET 0.092 μM (IC50) |
In Vitro
Flunamine (preincubation for 10 min; uptake incubation for 5 min) inhibits DA and 5-HT uptake in rat striatal synaptosomes with IC50 values of 0.26 μM and 0.17 μM, respectively[2].
Flunamine (preincubation for 10 min; uptake incubation for 5 min) inhibits the uptake of 5-HT and NA in rat hypothalamic synaptosomes, with IC50 values of 0.24 μM and 0.092 μM, respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 50366-32-0
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Molecular Weight 263.29
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Formula C15H15F2NO
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SMILES
FC1=CC=C(C=C1)C(OCCN)C2=CC=C(F)C=C2
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Human pluripotent stem cell midbrain dopaminergic neuron differentiation
Human pluripotent stem cells are directed toward midbrain dopaminergic neurons by first inducing a neural floor-plate-like progenitor state, then patterning cells with ventralizing SHH signaling and midbrain/WNT-FGF cues, and finally maturing progenitors into neurons expressing dopaminergic markers such as TH, NURR1/NR4A2, PITX3, DAT/SLC6A3, VMAT2/SLC18A2, GIRK2/KCNJ6, FOXA2, LMX1A, and EN1. The main readouts are loss of pluripotency, acquisition of FOXA2+/LMX1A+ midbrain floor-plate progenitors, emergence of βIII-tubulin+/MAP2+ neurons, and production of TH+ dopaminergic neurons with molecular, dopamine-release, and electrophysiological features of midbrain dopaminergic identity.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Flunamine Related Classifications
HY-W367959 Related Classifications
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