Fluorescent brightener 71
Based on 1 Customer Validation
Fluorescent brightener 71 (FB71) is an inhibitor targeting deubiquitinases UCHL5 and USP14, as well as a CD40 ligand. Fluorescent brightener 71 blocks enzymatic activity, induces apoptosis, inhibits cell growth and triggers reactive oxygen species production. Meanwhile, Fluorescent brightener 71 upregulates the expression of oxidative stress-related genes gpx-4 and sod-4, and reversibly increases the protein levels of UCHL5 and USP14 through a feedback response. Fluorescent brightener 71 inhibits the growth, movement and reproductive capacity of Caenorhabditis elegans, and also exhibits concentration-dependent toxic effects. Fluorescent brightener 71 can be applied to scientific research in related fields such as breast cancer.
For research use only. We do not sell to patients.
- CAS No.: 16090-02-1
- Formula: C40H38N12Na2O8S2
- Molecular Weight:924.91
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Storage:
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
FB71 (2.5 μM; 3 h) potently inhibits the ubiquitin-binding activity of UCHL5 in MDA-MB-468 and MCF-7 human breast cancer cells, and completely inhibits the ubiquitin-binding activity of USP14 in MCF-7 cells[1].
FB71 (15 μM; 12 h) increases the protein expression levels of UCHL5 and USP14 in MDA-MB-468 and MCF-7 human breast cancer cells, and induces PARP cleavage (a marker of apoptosis)[1].
FB71 (1-25 μM; 24 h-72 h) inhibits the viability of MDA-MB-468 and MCF-7 human breast cancer cells in a dose- and time-dependent manner[1].
Fluorescent brightener 71 binds to human CD40 ligand, inducible nitric oxide synthase, and platelet factor 4 with high affinity[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MDA-MB-468, MCF-7
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Concentration:15 μM
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Incubation Time:12 h
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Result:Increased protein levels of UCHL5 and USP14 in both MDA-MB-468 and MCF-7 cells.
Induced cleavage of PARP from its full-length 116 kDa form to the 85 kDa apoptotic fragment in both cell lines.
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Cell Line:MDA-MB-468, MCF-7
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Concentration:1-25 μM
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Incubation Time:24 h; 48 h; 72 h
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Result:Inhibited cell proliferation in a dose- and time-dependent manner in both cell lines.
Achieved an IC50 value of 10 μM in MDA-MB-468 cells at 72 h.
Achieved an IC50 value of 9 μM in MCF-7 cells at 72 h.
Showed the highest potency among the tested optical brightener compounds.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Caenorhabditis elegans wild-type strain N2 (larval stages L1 and L4); transgenic strains gpx-4::GFP (BC20305), sod-4::GFP (BC20333), hsp-4::GFP (SJ4005)[2]
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Dosage:100 µM, 250 µM, 500 µM, 750 µM, 1000 µM, 5000 µM; 50-500 µM (transgenic reporter assay)
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Administration:exposure in K-medium; static incubation; 24 hours (lethality, locomotion, reproduction, transgenic reporter assay); 48 hours (body length)
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Result:Caused 3.6% lethality at 5000 µM, with significant increases at 2500 µM and 5000 µM compared to control.
Induced 52% growth inhibition at 1000 µM, with significant reductions observed at all tested concentrations (50 µM to 1000 µM) compared to control.
Reduced body bends over 20 seconds by 43% at 500 µM, with significant reductions observed at 250 µM and 500 µM compared to control.
Reduced egg-laying capacity by 51% at 500 µM, with significant reductions observed at 250 µM and 500 µM compared to control; caused a 7% reduction at 50 µM.
Increased relative expression of hsp-3 by 1.46-fold, hsp-4 by 1.39-fold, gpx-4 by 1.70-fold, and sod-4 by 1.70-fold at 500 µM.
Increased sod-4 expression by 1.45-fold at 100 µM and 1.40-fold at 250 µM.
Increased hsp-4 expression by 1.23-fold at 250 µM.
Exhibited a clear concentration-response relationship for all endpoints.
Chemical Information
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CAS No. 16090-02-1
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Appearance Solid
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Molecular Weight 924.91
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Formula C40H38N12Na2O8S2
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SMILES
O=S(C1=CC(NC2=NC(N3CCOCC3)=NC(NC4=CC=CC=C4)=N2)=CC=C1/C=C/C5=CC=C(NC6=NC(N7CCOCC7)=NC(NC8=CC=CC=C8)=N6)C=C5S(=O)(O[Na])=O)(O[Na])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)
Solvent & Solubility
H2O : 2 mg/mL (2.16 mM; ultrasonic and warming and heat to 60°C)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
References
[1]. Castro I, et al. Proteasome-associated cysteine deubiquitinases are molecular targets of environmental optical brightener compounds. J Cell Biochem. 2019;120(8):14065-14075. [Content Brief]
[2]. Castro-Sierra I, et al. Toxicity of Three Optical Brighteners: Potential Pharmacological Targets and Effects on Caenorhabditis elegans. Toxics. 2024 Jan 9;12(1):51. [Content Brief]
[3]. Chen H, et al. Identification of Fluorescent Brighteners as Another Emerging Class of Abundant, Ubiquitous Pollutants in the Indoor Environment. Environ Sci Technol. 2022;56(14):10131-10140. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| H2O | 1 mM | 1.0812 mL | 5.4059 mL | 10.8119 mL | 27.0297 mL |
* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.
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