2. Anti-infection Cell Cycle/DNA Damage Epigenetics
  3. Parasite Deubiquitinase PARP
  4. Fluorescent brightener 71

Fluorescent brightener 71 (FB71) is an inhibitor targeting deubiquitinases UCHL5 and USP14, as well as a CD40 ligand. Fluorescent brightener 71 blocks enzymatic activity, induces apoptosis, inhibits cell growth and triggers reactive oxygen species production. Meanwhile, Fluorescent brightener 71 upregulates the expression of oxidative stress-related genes gpx-4 and sod-4, and reversibly increases the protein levels of UCHL5 and USP14 through a feedback response. Fluorescent brightener 71 inhibits the growth, movement and reproductive capacity of Caenorhabditis elegans, and also exhibits concentration-dependent toxic effects. Fluorescent brightener 71 can be applied to scientific research in related fields such as breast cancer.

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Fluorescent brightener 71

Fluorescent brightener 71 Chemical Structure

CAS No. : 16090-02-1

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Based on 1 publication(s) in Google Scholar

Other Forms of Fluorescent brightener 71:

Fluorescent brightener 71 Related Antibodies

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PARP PARP1 PARP2 PARP3 PARP4 TNKS1/PARP5A TNKS2/PARP5B PARP7 PARP10 PARP14 PARP15 PARP12 PARP16

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Description

Fluorescent brightener 71 (FB71) is an inhibitor targeting deubiquitinases UCHL5 and USP14, as well as a CD40 ligand. Fluorescent brightener 71 blocks enzymatic activity, induces apoptosis, inhibits cell growth and triggers reactive oxygen species production. Meanwhile, Fluorescent brightener 71 upregulates the expression of oxidative stress-related genes gpx-4 and sod-4, and reversibly increases the protein levels of UCHL5 and USP14 through a feedback response. Fluorescent brightener 71 inhibits the growth, movement and reproductive capacity of Caenorhabditis elegans, and also exhibits concentration-dependent toxic effects. Fluorescent brightener 71 can be applied to scientific research in related fields such as breast cancer[1][2][3].

In Vitro

FB71 (2.5 μM; 3 h) potently inhibits the ubiquitin-binding activity of UCHL5 in MDA-MB-468 and MCF-7 human breast cancer cells, and completely inhibits the ubiquitin-binding activity of USP14 in MCF-7 cells[1].
FB71 (15 μM; 12 h) increases the protein expression levels of UCHL5 and USP14 in MDA-MB-468 and MCF-7 human breast cancer cells, and induces PARP cleavage (a marker of apoptosis)[1].
FB71 (1-25 μM; 24 h-72 h) inhibits the viability of MDA-MB-468 and MCF-7 human breast cancer cells in a dose- and time-dependent manner[1].
Fluorescent brightener 71 binds to human CD40 ligand, inducible nitric oxide synthase, and platelet factor 4 with high affinity[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Fluorescent brightener 71 Related Antibodies

Western Blot Analysis[1]

Cell Line: MDA-MB-468, MCF-7
Concentration: 15 μM
Incubation Time: 12 h
Result: Increased protein levels of UCHL5 and USP14 in both MDA-MB-468 and MCF-7 cells.
Induced cleavage of PARP from its full-length 116 kDa form to the 85 kDa apoptotic fragment in both cell lines.

Cell Viability Assay[1]

Cell Line: MDA-MB-468, MCF-7
Concentration: 1-25 μM
Incubation Time: 24 h; 48 h; 72 h
Result: Inhibited cell proliferation in a dose- and time-dependent manner in both cell lines.
Achieved an IC50 value of 10 μM in MDA-MB-468 cells at 72 h.
Achieved an IC50 value of 9 μM in MCF-7 cells at 72 h.
Showed the highest potency among the tested optical brightener compounds.
In Vivo

Fluorescent brightener 71 (50-5000 µM; exposure in K-medium; static incubation; 24-48 hours) induces low lethality but significant, concentration-dependent reductions in growth, locomotion, and reproduction in Caenorhabditis elegans, alongside upregulation of oxidative and cellular stress-related genes[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Caenorhabditis elegans wild-type strain N2 (larval stages L1 and L4); transgenic strains gpx-4::GFP (BC20305), sod-4::GFP (BC20333), hsp-4::GFP (SJ4005)[2]
Dosage: 100 µM, 250 µM, 500 µM, 750 µM, 1000 µM, 5000 µM; 50-500 µM (transgenic reporter assay)
Administration: exposure in K-medium; static incubation; 24 hours (lethality, locomotion, reproduction, transgenic reporter assay); 48 hours (body length)
Result: Caused 3.6% lethality at 5000 µM, with significant increases at 2500 µM and 5000 µM compared to control.
Induced 52% growth inhibition at 1000 µM, with significant reductions observed at all tested concentrations (50 µM to 1000 µM) compared to control.
Reduced body bends over 20 seconds by 43% at 500 µM, with significant reductions observed at 250 µM and 500 µM compared to control.
Reduced egg-laying capacity by 51% at 500 µM, with significant reductions observed at 250 µM and 500 µM compared to control; caused a 7% reduction at 50 µM.
Increased relative expression of hsp-3 by 1.46-fold, hsp-4 by 1.39-fold, gpx-4 by 1.70-fold, and sod-4 by 1.70-fold at 500 µM.
Increased sod-4 expression by 1.45-fold at 100 µM and 1.40-fold at 250 µM.
Increased hsp-4 expression by 1.23-fold at 250 µM.
Exhibited a clear concentration-response relationship for all endpoints.
Molecular Weight

924.91

Formula

C40H38N12Na2O8S2
CAS No.
Appearance

Solid

SMILES

O=S(C1=CC(NC2=NC(N3CCOCC3)=NC(NC4=CC=CC=C4)=N2)=CC=C1/C=C/C5=CC=C(NC6=NC(N7CCOCC7)=NC(NC8=CC=CC=C8)=N6)C=C5S(=O)(O[Na])=O)(O[Na])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, stored under nitrogen

*In solvent : -80°C, 6 months; -20°C, 1 month (stored under nitrogen)

Solvent & Solubility
In Vitro: 

H2O : 2 mg/mL (2.16 mM; ultrasonic and warming and heat to 60°C)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.0812 mL 5.4059 mL 10.8119 mL
5 mM --- --- ---
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
H2O 1 mM 1.0812 mL 5.4059 mL 10.8119 mL 27.0297 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

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  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Fluorescent brightener 7116090-02-1Fluorescent brightener71Fluorescent brightener-71ParasiteDeubiquitinasePARPDUBspoly ADP ribose polymerasereactive oxygen speciessod-4gpx-4MDA-MB-468UCHL5apoptosisCD40 ligandCaenorhabditis elegansUSP14MCF-7Inhibitorinhibitorinhibit

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