Asperphenamate
Based on 1 Customer Validation
Asperphenamate, a fungal metabolite of Aspergillus flatiipes with anti-cancer effect, exhibits IC50 values of 92.3 μM, 96.5 μM and 97.9 μM in T47D, MDA-MB-231 and HL-60 cells, respectively.
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- Pureza : 98.0%
- No. CAS: 63631-36-7
- Fòrmula: C32H30N2O4
- Peso molecular:506.59
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| Bel-7402 | IC50 |
>100 μM
Compound: 1a
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Antiproliferative activity against human Bel7402 cells after 3 days by MTT assay
Antiproliferative activity against human Bel7402 cells after 3 days by MTT assay
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[PMID: 22361137] |
| HeLa | IC50 |
>100 μM
Compound: 1a
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Antiproliferative activity against human HeLa cells after 3 days by MTT assay
Antiproliferative activity against human HeLa cells after 3 days by MTT assay
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[PMID: 22361137] |
| MCF7 | IC50 |
94.76 μM
Compound: 1a
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Antiproliferative activity against human MCF7 cells after 3 days by MTT assay
Antiproliferative activity against human MCF7 cells after 3 days by MTT assay
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[PMID: 22361137] |
In Vitro
Asperphenamate can inhibit cancer cell proliferation by fully inducing autophagy. asperphenamate showed inhibition effects against cathepsin L. At the same time, it also displayed weak inhibitory ability against cathepsin S.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 63631-36-7
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Appearance Solid
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Peso molecular 506.59
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Fòrmula C32H30N2O4
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Color White to off-white
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SMILES
O=C(OC[C@@H](NC(C1=CC=CC=C1)=O)CC2=CC=CC=C2)[C@H](CC3=CC=CC=C3)NC(C4=CC=CC=C4)=O
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Structure Classification
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Initial Source
Aspergillus flavipes
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (197.40 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Instrucciones de manejo (2659 KB)
Referencias
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9740 mL | 9.8699 mL | 19.7398 mL | 49.3496 mL |
| 5 mM | 0.3948 mL | 1.9740 mL | 3.9480 mL | 9.8699 mL | |
| 10 mM | 0.1974 mL | 0.9870 mL | 1.9740 mL | 4.9350 mL | |
| 15 mM | 0.1316 mL | 0.6580 mL | 1.3160 mL | 3.2900 mL | |
| 20 mM | 0.0987 mL | 0.4935 mL | 0.9870 mL | 2.4675 mL | |
| 25 mM | 0.0790 mL | 0.3948 mL | 0.7896 mL | 1.9740 mL | |
| 30 mM | 0.0658 mL | 0.3290 mL | 0.6580 mL | 1.6450 mL | |
| 40 mM | 0.0493 mL | 0.2467 mL | 0.4935 mL | 1.2337 mL | |
| 50 mM | 0.0395 mL | 0.1974 mL | 0.3948 mL | 0.9870 mL | |
| 60 mM | 0.0329 mL | 0.1645 mL | 0.3290 mL | 0.8225 mL | |
| 80 mM | 0.0247 mL | 0.1234 mL | 0.2467 mL | 0.6169 mL | |
| 100 mM | 0.0197 mL | 0.0987 mL | 0.1974 mL | 0.4935 mL |
Asperphenamate Related Classifications
HY-129578 Related Classifications
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