Inact-dIRF4-2
Inact-dIRF4-2 is an inactive control compound of dIRF4-2 (HY-185697). Inact-dIRF4-2 lacks IRF4-binding activity and IRF4-dependent cytotoxic activity. Inact-dIRF4-2 can be used in the research of multiple myeloma.
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- No. CAS: 3136609-65-6
- Fòrmula: C43H46N8O7
- Peso molecular:786.87
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Almacenamiento:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| HEK-293T | IC50 |
50 μM
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Cytotoxicity against human HEK293T cells assessed as cell viability incubated for 96 hrs by CellTiter-Glo assay, with IC50 greater than 50 μM.
Cytotoxicity against human HEK293T cells assessed as cell viability incubated for 96 hrs by CellTiter-Glo assay, with IC50 greater than 50 μM.
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42209806 |
In Vitro
Inact-dIRF4-2 (96 h) does not induce cytotoxicity in 11 of 12 tested cell lines (including 11 IRF4-dependent multiple myeloma cell lines and the IRF4-independent HEK293T cell line), with IC50 values >50 μM, confirming IRF4-dependent cell killing by the active degrader dIRF4-2[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Karpas-707, RPMI-8226, JIN-3, MM1.S, AMO1, KMS-12-BM, OPM-2, KMS-11, LP-1, EJM, U266B1, MOLP-2, HEK293T
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Concentration:>50 μM
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Incubation Time:96 h
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Result:Showed no cytotoxic effects in 11 out of 12 tested cell lines, with IC50 values greater than 50 μM across all doses.
Chemical Information
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No. CAS 3136609-65-6
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Peso molecular 786.87
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Fòrmula C43H46N8O7
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SMILES
COC1=CC=C(C2(CC2)C(N[C@@H](C(NC)=O)C3=CC=CC(C4=CN(CCCN5CCN(C6=CC=C(C(N(C7C(NC(CC7)=O)=O)C8=O)=O)C8=C6)CC5)N=C4)=C3)=O)C=C1
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocolo
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Pureza y Documentación
Referencias
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Inact-dIRF4-2 Related Classifications
HY-185697A Related Classifications
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