Methisazone
Based on 1 Customer Validation
Methisazone (Marboran) is an antiviral agent that works by inhibiting mRNA and protein synthesis. Methisazone is also a SARS-CoV-2 (COVID-19) inhibitor. Methisazone is mainly used in pox viruses.
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- Pureza: 99.37%
- No. CAS: 1910-68-5
- Fòrmula: C10H10N4OS
- Peso molecular:234.28
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Almacenamiento:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Actividad biológica
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Chemical Information
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Solvente y solubilidad
- Pureza y Documentación
- Referencias
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Help & FAQs
Actividad biológica
Descripciòn
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| HFF | CC50 |
>427 μM
Compound: 2 ; Methisazone
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Half-maximal cytotoxic concentration in human foreskin fibroblast (HFF) cells
Half-maximal cytotoxic concentration in human foreskin fibroblast (HFF) cells
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[PMID: 15828843] |
| HFF | EC50 |
14 μM
Compound: 2 ; Methisazone
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Effective concentration required to inhibit cowpox virus plaque formation in human foreskin fibroblast (HFF) cells
Effective concentration required to inhibit cowpox virus plaque formation in human foreskin fibroblast (HFF) cells
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[PMID: 15828843] |
| HFF | EC50 |
3.3 μM
Compound: 2 ; Methisazone
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Effective concentration required to inhibit vaccinia virus plaque formation in human foreskin fibroblast (HFF) cells
Effective concentration required to inhibit vaccinia virus plaque formation in human foreskin fibroblast (HFF) cells
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[PMID: 15828843] |
In Vitro
The EC50 values of methisazone against six vaccinia viruses (Copenhagen, WR, NYC, Elstree, IHD and IHD) in vitro are 3.3, 0.06, 0.3, 0.5, 0.12 and 0.11 μM[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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No. CAS 1910-68-5
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Appearance Solid
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Peso molecular 234.28
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Fòrmula C10H10N4OS
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Color Light yellow to yellow
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SMILES
O=C1N(C2=C(/C1=N/NC(N)=S)C=CC=C2)C
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Synonyms
Marboran
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Envío
Room temperature in continental US; may vary elsewhere.
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Almacenamiento
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvente y solubilidad
In Vitro:
DMSO : 100 mg/mL (426.84 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Pureza y Documentación
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Ficha de datos (276 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Instrucciones de manejo (2659 KB)
Referencias
[1]. Bhumi Shah, et al. In silico studies on therapeutic agents for COVID-19: Drug repurposing approach. Life Sci. 2020 Jul 1;252:117652. [Content Brief]
[2]. Smee DF, et, al. Progress in the discovery of compounds inhibiting orthopoxviruses in animal models. Antivir Chem Chemother. 2008;19(3):115-24. [Content Brief]
[3]. Quenelle D C, et al. In vitro and in vivo evaluation of isatin-β-thiosemicarbazone and marboran against vaccinia and cowpox virus infections[J]. Antiviral Research, 2006, 71(1): 24-30. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 4.2684 mL | 21.3420 mL | 42.6840 mL | 106.7099 mL |
| 5 mM | 0.8537 mL | 4.2684 mL | 8.5368 mL | 21.3420 mL | |
| 10 mM | 0.4268 mL | 2.1342 mL | 4.2684 mL | 10.6710 mL | |
| 15 mM | 0.2846 mL | 1.4228 mL | 2.8456 mL | 7.1140 mL | |
| 20 mM | 0.2134 mL | 1.0671 mL | 2.1342 mL | 5.3355 mL | |
| 25 mM | 0.1707 mL | 0.8537 mL | 1.7074 mL | 4.2684 mL | |
| 30 mM | 0.1423 mL | 0.7114 mL | 1.4228 mL | 3.5570 mL | |
| 40 mM | 0.1067 mL | 0.5335 mL | 1.0671 mL | 2.6677 mL | |
| 50 mM | 0.0854 mL | 0.4268 mL | 0.8537 mL | 2.1342 mL | |
| 60 mM | 0.0711 mL | 0.3557 mL | 0.7114 mL | 1.7785 mL | |
| 80 mM | 0.0534 mL | 0.2668 mL | 0.5335 mL | 1.3339 mL | |
| 100 mM | 0.0427 mL | 0.2134 mL | 0.4268 mL | 1.0671 mL |
Methisazone Related Classifications
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