Referencia número: HY-125506

Vopenfasinib

Name: Vopenfasinib
Brand: MedChemExpress
SKU: HY-125506
Price: 62.00 USD
Availability: InStock
Rating: 5 (8 reviews)

(Synonyms: NP-G2-044)

8 Cited Publications

Revisión del cliente

Based on 8 publication(s) in Google Scholar

NP-G2-044 is a potent, orally active fascin inhibitor, with an IC₅₀ of ~2 μM. NP-G2-044 blocks tumor metastasis and increases antitumor immune response.

Para uso exclusivo en investigación. No vendemos a pacientes.

Pureza : 99.78%
No. CAS: 1807454-59-6
Fòrmula: C₂₁H₁₆F₃N₃O₂
Peso molecular:399.37

Almacenamiento:

Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 2 years , -20°C, 1 year

Vopenfasinib Chemical Structure

No. CAS: 1807454-59-6

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Top Publications Citing

Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- In Vitro
- In Vivo
- Ensayo clínico
Chemical Information
Publications (8)
Solvente y solubilidad
- In Vitro
- In Vivo
Protocolo (2)
Pureza y Documentación
Referencias
Help & FAQs
Classifications
Bioz

Actividad biológica

Descripciòn

NP-G2-044 is a potent, orally active fascin inhibitor, with an IC₅₀ of ~2 μM. NP-G2-044 blocks tumor metastasis and increases antitumor immune response^[1]^[2].

In Vitro

NP-G2-044 inhibits the migration of MDA-MB-231 tumor cells with an IC₅₀ ~10 μM. NP-G2-044 decreases filopodial formation resulting in decreased lamellipodial formation^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

In Vivo

NP-G2-044 (100 mg/kg; i.p.; daily for 18 days) inhibits breast tumor metastasis in mouse models^[1].
Upon oral administration, NP-G2-044 targets and binds to fascin, thereby preventing the interaction of fascin with actin filaments, thereby preventing actin bundling and filopodia formation. By preventing actin cytoskeletal reorganization, the dynamic changes in cell shape that are necessary for tumor cell migration and invasion to occur are impaired, and tumor cell migration and metastasis are inhibited^[3].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:

6-8 week old female BALB/c mice (mouse model bearing 4T1 tumor cells)^[1]
Dosage:

100 mg/kg
Administration:

I.p.; daily for 18 days
Result:

Inhibition of 4T1 mouse mammary tumor cell metastasis to the lung.

Ensayo clínico

NCT Number	Sponsor	Condition	Start Date	Phase A549
NCT01329991	Plexxikon\|		2011-05	PHASE1

Chemical Information

No. CAS 1807454-59-6
Appearance Solid
Peso molecular 399.37
Fòrmula C₂₁H₁₆F₃N₃O₂
Color Off-white to light yellow
SMILES

O=C(C1=C(C)OC=C1)NC2=NN(CC3=CC=C(C(F)(F)F)C=C3)C4=C2C=CC=C4
Synonyms

NP-G2-044
Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	2 years
	-20°C	1 year

Publications (8)

All IFWBCell Imaging/StainingRT-PCRIn Vivo Efficacy Study

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Journal Impact Factor

Bone Res

Targeting Fascin1 maintains chondrocytes phenotype and attenuates osteoarthritis development. [Abstract]

2024 Sep 4;12(1):50. PMID: 39231936

Vopenfasinib purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Sep 4;12(1):50. [Abstract]

NP-G2-044 (NP; 10 μM; 24 h) inhibited IL-1β (10 mg/mL) enhanced the interaction between FSCN1 and F-actin mouse chondrocytes.

Vopenfasinib purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Sep 4;12(1):50. [Abstract]

NP-G2-044 (NP; 10 μM; 24 h) inhibited the transition of G-actin to F-actin and the flattened cell shape caused by IL-1β (10 mg/mL) stimulation, maintaining a more rounded morphology in mouse chondrocytes.

Vopenfasinib purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Sep 4;12(1):50. [Abstract]

Alcian blue staining showed that NP-G2-044 (NP; 10 μM; 24 h) treatment preserved proteoglycan content despite IL-1β (10 mg/mL) stimulation in mouse chondrocytes.

Vopenfasinib purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Sep 4;12(1):50. [Abstract]

NP-G2-044 (NP; 10 μM; 24 h)markedly increased Col2 and decreased Col3 expression compared to the IL-1β-treated group in mouse chondrocytes.

Vopenfasinib purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Sep 4;12(1):50. [Abstract]

NP-G2-044 (NP; 10 μM; 24 h) up-regulated chondrogenic genes and down-regulated dedifferentiation genes in mouse chondrocytes.

Vopenfasinib purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Sep 4;12(1):50. [Abstract]

NP-G2-044 (NP; 10 μM; 24 h) rescued the chondrocytes from dedifferentiation after IL-1β stimulation, increased Col2 and DCN expression, decreased Col3 expression, and inhibited ALK1/Smad1/5 signaling in mouse chondrocytes.

Vopenfasinib purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Sep 4;12(1):50. [Abstract]

NP-G2-044 (NP; 10 mM for 10 μL; once a week via intra-articular administration into the right knee) showed the expression of chondrocyte catabolic markers (MMP3, MMP13), dedifferentiation markers (Col3, Col1), p-Smad1/5, and β-catenin significantly decreased, while the expression of chondrocyte anabolism markers (Col2a1, Acan) increased in mice.

Vopenfasinib purchased from MedChemExpress. Usage Cited in: Bone Res. 2024 Sep 4;12(1):50. [Abstract]

NP-G2-044 (NP; 10 μM; 24 h) revealed inhibition of FSCN1 and decreased binding to F-actin in human articular chondrocytes.
Adv Sci (Weinh)

ERM Inhibition Confers Ferroptosis Resistance through ROS-Induced NRF2 Signaling. [Abstract]

2026 Mar;13(16):e13310. PMID: 41589654
Adv Sci (Weinh)

Low YTHDC1 Expression Upregulates FSCN1 to Promote Nuclear F-Actin Formation and Facilitate Double-strand DNA Breaks Repair in TMZ-Resistant Glioblastoma. [Abstract]

2025 Dec 27:e13632. PMID: 41454694
Cell Death Dis

Fascin enhances the vulnerability of breast cancer to erastin-induced ferroptosis. [Abstract]

2022 Feb 14;13(2):150. PMID: 35165254
J Transl Med

Targeting FSCN1 with an oral small-molecule inhibitor for treating ocular neovascularization. [Abstract]

2023 Aug 18;21(1):555. PMID: 37596693
Curr Biol

Regulation of mitophagy by Fis1 and Fascin1-organized actin. [Abstract]

2026 Feb 24:S0960-9822(26)00134-X. PMID: 41742405
bioRxiv

A Critical Role for the Fascin Family of Actin Bundling Proteins in Axon Development, Brain Wiring and Function. [Abstract]

2025 Jun 10:2025.02.21.639554. PMID: 40027761
bioRxiv

Anchorage-independent cell proliferation promoted by fascin's F-actin bundling. [Abstract]

2024 May 4. PMID: 38746129

Solvente y solubilidad

In Vitro:

DMSO : 100 mg/mL (250.39 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

View the Complete Stock Solution Preparation Table

Preparing Stock Solutions

View the Complete Stock Solution Preparation Table

Concentration Solvent Mass

1 mg

5 mg

10 mg

Concentration

Solvent

Mass

1 mM

2.5039 mL

12.5197 mL

25.0394 mL

5 mM

0.5008 mL

2.5039 mL

5.0079 mL

10 mM

0.2504 mL

1.2520 mL

2.5039 mL

Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

Stock Solution Working Solution

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline

Solubility: ≥ 2.5 mg/mL (6.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.

Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil

Solubility: ≥ 2.5 mg/mL (6.26 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage Dosage
mg/kg
Animal Weight (Per Animal) Animal Weight (Per Animal)
g
Dosing Volume (Per Animal) Dosing Volume (Per Animal)
μL
Number of Animals Number of Animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

%
DMSO +

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
%
+
+
%
Tween-80 + +
%
Saline +

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Working solution concentration: 0.22 mg/mL

Protocolo

All Cell Biology Animals and Diseases

Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Orthotopic Cell-Line Xenograft
Orthotopic cell-line xenograft models involve implantation of human cancer cell lines into the anatomically corresponding organ of immunodeficient mice to reproduce tumor growth within a native microenvironment, enabling more clinically relevant tumor behavior compared with subcutaneous models. These models are widely used because orthotopic placement better recapitulates tumor progression, including invasion and metastatic spread, which are often underrepresented in heterotopic implantation systems. Compared with conventional xenografts, orthotopic implantation is described as more technically complex but provides improved simulation of tumor-microenvironment interactions and metastatic behavior, making it particularly valuable for translational oncology research. Surgical orthotopic implantation approaches have been emphasized as enabling faithful reproduction of clinical cancer features, including metastasis and disease progression patterns that align with the tumor’s organ of origi

Pureza y Documentación

Select Batch:

Pureza: 99.98%

Assay: 99.98%

ee.: 99.98%

Ficha de datos (281 KB)
SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instrucciones de manejo (2659 KB)

Referencias

[1]. Han S, et al. Improving fascin inhibitors to block tumor cell migration and metastasis. Mol Oncol. 2016;10(7):966-980. [Content Brief]

[2]. Vincent Chung, et al. Abstract C053: NP-G2-044, a novel fascin inhibitor, blocks tumor metastasis and increases antitumor immune response.

[3]. Fascin Inhibitor NP-G2-044.

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Complete Stock Solution Preparation Table

Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg
DMSO	1 mM	2.5039 mL	12.5197 mL	25.0394 mL	62.5986 mL
	5 mM	0.5008 mL	2.5039 mL	5.0079 mL	12.5197 mL
	10 mM	0.2504 mL	1.2520 mL	2.5039 mL	6.2599 mL
	15 mM	0.1669 mL	0.8346 mL	1.6693 mL	4.1732 mL
	20 mM	0.1252 mL	0.6260 mL	1.2520 mL	3.1299 mL
	25 mM	0.1002 mL	0.5008 mL	1.0016 mL	2.5039 mL
	30 mM	0.0835 mL	0.4173 mL	0.8346 mL	2.0866 mL
	40 mM	0.0626 mL	0.3130 mL	0.6260 mL	1.5650 mL
	50 mM	0.0501 mL	0.2504 mL	0.5008 mL	1.2520 mL
	60 mM	0.0417 mL	0.2087 mL	0.4173 mL	1.0433 mL
	80 mM	0.0313 mL	0.1565 mL	0.3130 mL	0.7825 mL
	100 mM	0.0250 mL	0.1252 mL	0.2504 mL	0.6260 mL

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Vopenfasinib

8 Cited Publications

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Vopenfasinib

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