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Referencia número: HY-N19214

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Glenthmycin L

Glenthmycin L is a potent antibiotic that can be found in Australian sheep pasture-derived Streptomyces sp. CMB-PB041. Glenthmycin L exhibits antibacterial activity against Gram-positive bacterial pathogens including Staphylococcus aureus, Enterococcus faecalis, Methicillin (HY-B0974)-resistant Staphylococcus aureus, and Vancomycin (HY-B0671)-resistant Enterococci. Glenthmycin L can be used for the research of bacterial infections caused by Gram-positive pathogens.

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No. CAS: 3040055-91-9
Fòrmula: C₃₅H₄₆O₆
Peso molecular:562.75

Almacenamiento:

Please store the product under the recommended conditions in the Certificate of Analysis.

Glenthmycin L Chemical Structure

No. CAS: 3040055-91-9

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Use of MCE Products

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Actividad biológica
- Descripciòn
- Cellular Effect
- In Vitro
Chemical Information
Protocolo (3)
Pureza y Documentación
Referencias
Calculators
Help & FAQs
Classifications
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Actividad biológica

Descripciòn

Ver todos los productos específicos de isoformas Antibiotic :

Cellular Effect

Cell Line	Type	Value	Description	References
NCI-H460	IC₅₀	30 μM Compound: 12	Cytotoxicity against human NCI-H460 cells and measured after 48 hrs by MTT assay Cytotoxicity against human NCI-H460 cells and measured after 48 hrs by MTT assay	[PMID: 35640100]
SW-620	IC₅₀	30 μM Compound: 12	Cytotoxicity against human SW-620 cells and measured after 48 hrs by MTT assay Cytotoxicity against human SW-620 cells and measured after 48 hrs by MTT assay	[PMID: 35640100]

In Vitro

Glenthmycin L (0.01-30 μM; 16-24 h) potently inhibits growth of Staphylococcus aureus (ATCC 25923) with an IC₅₀ of 0.8 μM, methicillin-resistant S. aureus (ATCC 43300) with an IC₅₀ of 0.3 μM, Enterococcus faecalis with an IC₅₀ of 3.0 μM, methicillin-resistant S. aureus (ATCC 33591) with an IC₅₀ of 6.8 μM, and vancomycin-resistant Enterococci with an IC₅₀ of 6.7 μM^[1].
Glenthmycin L (30 μM; 48 h) shows no significant cytotoxicity against SW620 human colorectal carcinoma cells or NCI-H460 human lung carcinoma cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.

Cell Cytotoxicity Assay^[1]

View Protocol

Cell Line:

SW620 human colorectal carcinoma cells, NCI-H460 human lung carcinoma cells
Concentration:

30 μM
Incubation Time:

48 h
Result:

Did not exhibit significant cytotoxicity against SW620 or NCI-H460 cells.
Showed no indication of reduced cell viability relative to the negative control.

Chemical Information

No. CAS 3040055-91-9
Peso molecular 562.75
Fòrmula C₃₅H₄₆O₆
SMILES

OC=1[C@@]23[C@@](C=C(C)[C@H](C)C2)(/C=C(\C)/CC/C=C(\C)/[C@]4([C@@](C)(C(=O)C1C(=O)O3)[C@]5([C@@]([C@@H](OC(C)=O)CC[C@@H]5C)(C=C4C)[H])[H])[H])[H]
Structure Classification
- Antibiotics
- Other Antibiotics
Initial Source
- Microorganisms
Streptomyces sp.
Envío

Room temperature in continental US; may vary elsewhere.
Almacenamiento

Please store the product under the recommended conditions in the Certificate of Analysis.

Protocolo

All Signal Pathway Related Histomorphology Cell Biology

Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
Gram Staining of Tissue Sections
Gram staining of tissue sections is a histochemical technique used to differentiate Gram-positive and Gram-negative bacteria within histological specimens based on differences in bacterial cell wall structure and dye retention, adapted from classical bacteriological Gram staining into tissue-compatible “histological Gram stain” variants. In tissue applications, modifications of the Brown-Hopps and Brown-Brenn methods are commonly used to improve differentiation of microorganisms embedded within host connective tissue and to reduce overstaining or loss of Gram-negative signal, which are known limitations of earlier approaches. The principle relies on crystal violet-iodine complex retention in Gram-positive organisms and subsequent decolorization and counterstaining steps that allow contrast visualization of Gram-negative organisms against tissue background.
Bacterial live/dead nucleic-acid viability staining
The LIVE/DEAD bacterial viability staining method is based on differential permeability of nucleic-acid-binding fluorescent dyes, most commonly SYTO 9 and propidium iodide (PI), which enables discrimination of bacterial populations with intact versus compromised cytoplasmic membranes. SYTO 9 penetrates both intact and damaged bacterial membranes and binds nucleic acids to produce green fluorescence, whereas propidium iodide penetrates only cells with compromised membranes and fluoresces red while also reducing SYTO 9 signal through competitive binding and fluorescence interactions. The resulting fluorescence pattern is interpreted as a proxy for membrane integrity, which is widely used as an indicator of bacterial viability in microscopy, flow cytometry, and spectroscopic platforms. However, mechanistic studies show that SYTO 9 and PI interactions involve displacement and fluorescence resonance energy transfer effects, which can influence signal interpretation depending on dye ratios a

Pureza y Documentación

Instrucciones de manejo (2659 KB)

Referencias

[1]. Wu T, et al. Glenthmycins A-M: Macrocyclic Spirotetronate Polyketide Antibacterials from the Australian Pasture Plant-Derived Streptomyces sp. CMB-PB041. J Nat Prod. 2022 Jun 24;85(6):1641-1657. [Content Brief]

Calculators

Calculadora de molaridad

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Molecular Weight Molecular Weight

Calculadora de dilución

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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100 mg

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