Glucocamelinin
Glucocamelinin (GSL 10) is a sulfinyl glucosinolate identified from seeds of Camelina sativa and an upregulator of quinone reductase (NQO1). Glucocamelinin upregulates the activity of phase II detoxifying enzymes, exhibits selective cytotoxic activity against lung cancer cells, and possesses in vitro antioxidant activity. Glucocamelinin can be used in studies related to non-small cell lung cancer.
For research use only. We do not sell to patients.
- CAS No.: 67884-10-0
- Formula: C18H35NO10S3
- Molecular Weight:521.67
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
NQO1 |
In Vitro
Glucocamelinin (1.25-5 μM; 24 h) shows no cytotoxicity at concentrations of 1.25 μM and 2.5 μM in Hepa1c1c7 mouse hepatocellular carcinoma cells, but reduces cell viability to approximately 70% of that in the control group at a concentration of 5 μM[1].
Glucocamelinin (1.25-5 μM; 24 h) dose-dependently induces NQO1 activity in Hepa1c1c7 mouse hepatocellular carcinoma cells in vitro[1].
Glucocamelinin (1.25-5 μM; 24 h) does not induce or inhibit the catalytic activity of CYP1A1 in Hepa1c1c7 mouse hepatocellular carcinoma cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:Hepa1c1c7 mouse hepatoma cells
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Concentration:1.25, 2.5, 5 μM
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Incubation Time:24 h
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Result:Showed no cytotoxicity relative to untreated controls at 1.25 μM and 2.5 μM.
Reduced cell survival to approximately 70% of the untreated control at 5 μM.
Chemical Information
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CAS No. 67884-10-0
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Molecular Weight 521.67
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Formula C18H35NO10S3
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SMILES
CS(CCCCCCCCCC/C(S[C@@H]1O[C@@H]([C@H]([C@@H]([C@H]1O)O)O)CO)=N/OS(=O)(O)=O)=O
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Synonyms
10-(Methylsulfinyl)decylglucosinolate; GSL 10
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Glucocamelinin Related Classifications
HY-N20735 Related Classifications
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Keywords
- Glucocamelinin
- 67884-10-0
- 10-(Methylsulfinyl)decylglucosinolate
- GSL 10
- GSL10
- GSL 10
- GSL-10
- Quinone Reductase
- Reactive Oxygen Species (ROS)
- quinone reductase (NQO1)
- non-small-cell lung cancer
- phase II detoxification enzyme
- cytochrome P450 (CYP) 1A1
- Hepa1c1c7 mouse hepatoma cells
- quercetin
- lung cancer cells
- Camelina sativa
- sinigrin
- glucoarabin
- Inhibitor
- inhibitor
- inhibit