GPX4-IN-6

Name: GPX4-IN-6
Brand: MedChemExpress
SKU: HY-155664
Rating: 4.5 (0 reviews)

Cat. No.: HY-155664 Purity: 99.65%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

GPX4-IN-6 (Compound C25) is a GPX4 covalent inhibitor with an IC₅₀ value of 0.13 μM. GPX4-IN-6 (Compound C25) can induce ferroptosis for the research of triple-negative breast cancer (TNBC) .

연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.

GPX4-IN-6 화학구조

CAS No. : 2922824-07-3

사이즈	재고	수량
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	해외재고보유	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	해외재고보유	Estimated Time of Arrival: December 31
Solid
5 mg	해외재고보유	Estimated Time of Arrival: December 31
10 mg	해외재고보유	Estimated Time of Arrival: December 31
25 mg	해외재고보유	Estimated Time of Arrival: December 31
50 mg	해외재고보유	Estimated Time of Arrival: December 31
100 mg	해외재고보유	Estimated Time of Arrival: December 31
200 mg	견적 받기
500 mg	견적 받기

or 대량구매 문의

* 장바구니에 담기 전 물품의 수량을 선택해 주십시오.

This product is a controlled substance and not for sale in your territory.

고객리뷰

Based on 1 Customer Validation

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View All Isoforms

GPX1 GPX4

Biological Activity
순도&문서
References
고객리뷰

제품 설명

IC₅₀ & Target

GPX4

Cellular Effect

Cell Line	Type	Value	Description	References
BT-549	IC₅₀	0.034 μM Compound: C25	Antiproliferative activity against human BT-549 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human BT-549 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay	[PMID: 37452764]
MDA-MB-231	IC₅₀	0.008 μM Compound: C25	Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay Antiproliferative activity against human MDA-MB-231 cells assessed as inhibition of cell viability incubated for 72 hrs by MTT assay	[PMID: 37452764]
MDA-MB-231	IC₅₀	0.018 μM Compound: C25	Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of autophagy inhibitor, 3-MA by MTT assay Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of autophagy inhibitor, 3-MA by MTT assay	[PMID: 37452764]
MDA-MB-231	IC₅₀	0.025 μM Compound: C25	Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of necrosis inhibitor, necrostatin-1 by MTT assay Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of necrosis inhibitor, necrostatin-1 by MTT assay	[PMID: 37452764]
MDA-MB-231	IC₅₀	0.03 μM Compound: C25	Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of apoptosis inhibitor, Z-VAD-FMK by MTT assay Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of apoptosis inhibitor, Z-VAD-FMK by MTT assay	[PMID: 37452764]
MDA-MB-231	IC₅₀	1.75 μM Compound: C25	Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of ferroptosis inhibitor, ferrostatin-1 by MTT assay Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of ferroptosis inhibitor, ferrostatin-1 by MTT assay	[PMID: 37452764]
MDA-MB-231	IC₅₀	1.85 μM Compound: C25	Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of deferoxamine by MTT assay Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of deferoxamine by MTT assay	[PMID: 37452764]
MDA-MB-231	IC₅₀	> 20 μM Compound: C25	Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of N-acetyl-L-cysteine by MTT assay Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of N-acetyl-L-cysteine by MTT assay	[PMID: 37452764]
MDA-MB-231	IC₅₀	> 20 μM Compound: C25	Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of glutathione by MTT assay Induction of ferroptosis in human MDA-MB-231 cells assessed as cell viability incubated for 72 hrs in presence of glutathione by MTT assay	[PMID: 37452764]
MDA-MB-468	IC₅₀	0.012 μM Compound: C25	Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell viability incubated for 72 hrs by CCK-8 assay Antiproliferative activity against human MDA-MB-468 cells assessed as inhibition of cell viability incubated for 72 hrs by CCK-8 assay	[PMID: 37452764]

분자량

426.23

화학식

C₁₈H₁₇BrFNO₅

CAS No.

2922824-07-3

Appearance

Solid

Color

White to off-white

SMILES

O=C(CBr)N(C1=CC(OC)=C(F)C(OC)=C1)C2=CC(OCCO3)=C3C=C2

선적

Room temperature in continental US; may vary elsewhere.

보관

Powder	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

용액&용해도

In Vitro:

DMSO : 50 mg/mL (117.31 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.3462 mL	11.7308 mL	23.4615 mL
5 mM	0.4692 mL	2.3462 mL	4.6923 mL
10 mM	0.2346 mL	1.1731 mL	2.3462 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

몰농도 계산기
농도 희석 계산기

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (5.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (5.87 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Please enter your animal formula composition:

DMSO +

Tween-80 +

Saline

Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.

The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).

Calculation results:

Working solution concentration: mg/mL

Method for preparing stock solution: mg drug dissolved in μL DMSO (Stock solution concentration: mg/mL).

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only. If necessary, please contact MedChemExpress (MCE).

Method for preparing in vivo working solution for animal experiments: Take μL DMSO stock solution, add μL . μL , mix evenly, next add μL Tween 80, mix evenly, then add μL Saline.

If the continuous dosing period exceeds half a month, please choose this protocol carefully.

Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.

순도&문서

Purity: 99.65%

Select Batch:

Data Sheet (276 KB) SDS (251 KB)

COA (255 KB) HNMR (264 KB) LCMS (95 KB)

Handling Instructions (2659 KB)

References

[1]. Chen T, et al. Discovery of Novel Potent Covalent Glutathione Peroxidase 4 Inhibitors as Highly Selective Ferroptosis Inducers for the Treatment of Triple-Negative Breast Cancer. J Med Chem. 2023 Jul 27;66(14):10036-10059. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.3462 mL	11.7308 mL	23.4615 mL	58.6538 mL
	5 mM	0.4692 mL	2.3462 mL	4.6923 mL	11.7308 mL
	10 mM	0.2346 mL	1.1731 mL	2.3462 mL	5.8654 mL
	15 mM	0.1564 mL	0.7821 mL	1.5641 mL	3.9103 mL
	20 mM	0.1173 mL	0.5865 mL	1.1731 mL	2.9327 mL
	25 mM	0.0938 mL	0.4692 mL	0.9385 mL	2.3462 mL
	30 mM	0.0782 mL	0.3910 mL	0.7821 mL	1.9551 mL
	40 mM	0.0587 mL	0.2933 mL	0.5865 mL	1.4663 mL
	50 mM	0.0469 mL	0.2346 mL	0.4692 mL	1.1731 mL
	60 mM	0.0391 mL	0.1955 mL	0.3910 mL	0.9776 mL
	80 mM	0.0293 mL	0.1466 mL	0.2933 mL	0.7332 mL
	100 mM	0.0235 mL	0.1173 mL	0.2346 mL	0.5865 mL

GPX4-IN-6 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

GPX4-IN-62922824-07-3Glutathione PeroxidaseFerroptosisglutathione peroxidase 4 (GPX4)triple-negative breast cancer (TNBC)ferroptosisInhibitorinhibitorinhibit

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GPX4-IN-6

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GPX4-IN-6 Related Antibodies

Featured Recommendations

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

View All Glutathione Peroxidase Isoform Specific Products:

Biological Activity

순도&문서

References

고객리뷰

몰농도 계산기

농도 희석 계산기

Complete Stock Solution Preparation Table

GPX4-IN-6 Related Classifications

Keywords:

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고객리뷰

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SAFETY DATA SHEET (SDS)

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