H-(Gly)3-Lys(N3)-OH hydrochloride
Based on 1 Customer Validation
H-(Gly)3-Lys(N3)-OH (hydrochloride) is a click chemistry reagent. Click chemistry has great potential for use in binding between nucleic acids, lipids, proteins, and other molecules, and has been used in many research fields because of its beneficial characteristics, including high yield, high specificity, and simplicity. It contains an azide group and can undergo copper-catalyzed azide-alkyne cycloaddition (CuAAc) with molecules containing alkyne groups. It can also undergo ring strain-promoted alkyne-azide cycloaddition (SPAAC) with molecules containing DBCO or BCN groups.
For research use only. We do not sell to patients.
- Purity : 99.84%
- CAS No.: 2737202-70-7
- Formula: C12H22ClN7O5
- Molecular Weight:379.80
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Chemical Information
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CAS No. 2737202-70-7
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Appearance Solid
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Molecular Weight 379.80
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Formula C12H22ClN7O5
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Color White to off-white
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SMILES
NCC(NCC(NCC(N[C@H](C(O)=O)CCCCN=[N+]=[N-])=O)=O)=O.Cl
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
In Vitro:
DMSO : 125 mg/mL (329.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
The EdU incorporation assay measures DNA synthesis by adding the thymidine analog 5-ethynyl-2′-deoxyuridine to cells or tissues, where it is incorporated into newly synthesized DNA during S phase. Incorporated EdU is detected by copper-catalyzed azide-alkyne cycloaddition, in which a fluorescent azide covalently reacts with the ethynyl group on EdU, allowing S-phase cells to be detected by fluorescence microscopy, flow cytometry, or high-content imaging. EdU detection does not require DNA denaturation or anti-BrdU antibody access, which preserves sample structure and improves compatibility with immunostaining and multiparameter cytometry compared with BrdU-based detection. EdU can be cytotoxic in a cell-type- and exposure-dependent manner, so pulse duration, concentration, and continuous-labeling designs should be validated for each cell type.
Purity & Documentation
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Data Sheet (269 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.6330 mL | 13.1648 mL | 26.3296 mL | 65.8241 mL |
| 5 mM | 0.5266 mL | 2.6330 mL | 5.2659 mL | 13.1648 mL | |
| 10 mM | 0.2633 mL | 1.3165 mL | 2.6330 mL | 6.5824 mL | |
| 15 mM | 0.1755 mL | 0.8777 mL | 1.7553 mL | 4.3883 mL | |
| 20 mM | 0.1316 mL | 0.6582 mL | 1.3165 mL | 3.2912 mL | |
| 25 mM | 0.1053 mL | 0.5266 mL | 1.0532 mL | 2.6330 mL | |
| 30 mM | 0.0878 mL | 0.4388 mL | 0.8777 mL | 2.1941 mL | |
| 40 mM | 0.0658 mL | 0.3291 mL | 0.6582 mL | 1.6456 mL | |
| 50 mM | 0.0527 mL | 0.2633 mL | 0.5266 mL | 1.3165 mL | |
| 60 mM | 0.0439 mL | 0.2194 mL | 0.4388 mL | 1.0971 mL | |
| 80 mM | 0.0329 mL | 0.1646 mL | 0.3291 mL | 0.8228 mL | |
| 100 mM | 0.0263 mL | 0.1316 mL | 0.2633 mL | 0.6582 mL |
H-(Gly)3-Lys(N3)-OH hydrochloride Related Classifications
HY-151782A Related Classifications
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