HBT-Fl-BnB
HBT-Fl-BnB is a fluorescent probe for the ratiometric detection of ONOO- in vitro and in vivo. HBT-Fl-BnB consists of an HBT core with Fl groups at the ortho and para positions responding to the zwitterionic excited-state intramolecular proton-transfer (zwitterionic ESIPT) process and a boronic acid pinacol ester with dual roles that block the zwitterionic ESIPT and recognize ONOO-.
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- Formel: C75H88BNO3S
- Molecular Weight:1094.38
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biologische Aktivität
Beschreibung
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Raw264.7 cells are continued with different concentrations of HBT-Fl-BnB solution (0, 5, 10, 15, 20, 30, 50 μM) in the cell culture incubator.
2. After incubation for 24 h, dual channels for the confocal microscope are used to image the cells treated with HBT-Fl-BnB: the first channel (430ch) λem = 415?515 nm and the second channel (583ch) λex = 405 nm and λem = 550-650 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. .
In Vivo
Eight-week-old female C57BL/6 mice were selected. HBT-Fl-BnB (50 μM, 200 μL) is injected through the tail vein at 30 min before imaging. Then, these mice were imaged with the small animal in vivo imaging systems PerkinElmer IVIS Lumina XR and the Fluoroscopic Navigator 360I System. Dual channels for small animal in vivo imaging systems were used to image the mouse treated with HBT-Fl-BnB: the first channel (430ch) λem=415-515 nm and the second channel (583ch) λex=405 nm and λem=550-650 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Molecular Weight 1094.38
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Formel C75H88BNO3S
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SMILES
CC(O1)(C)C(C)(C)OB1C(C=C2)=CC=C2OC3=C(C=C(C4=CC(C(C=CC=C5)=C5C6(CCCCCC)CCCCCC)=C6C=C4)C=C3C7=CC(C(C=CC=C8)=C8C9(CCCCCC)CCCCCC)=C9C=C7)C%10=NC%11=C(S%10)C=CC=C%11
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Reinheit & Dokumentation
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
HBT-Fl-BnB Related Classifications
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