Herbacitrin
Herbacitrin is a HIV-1 reverse transcriptase and integrase inhibitor with IC50 values of 21.5 μM and 2.15 μM, respectively. Herbacitrin inhibits the growth of normal mouse embryonic cells, hybridoma HF cells, and ras/myc-transformed SFME cells, regulates the growth of ras-transformed SFME cells, and exhibits cytotoxic activity against hybridoma HF cells. Herbacitrin can be used in studies related to HIV-1 infection.
For research use only. We do not sell to patients.
- CAS No.: 35815-07-7
- Formula: C21H20O12
- Molecular Weight:464.38
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
HIV-1 |
In Vitro
Herbacitrin (4 days) exhibits dose-dependent cytotoxicity toward MT-4 and MT-2 human T-cell lines, with CC50 values of 27.8 μM and 63.64 μM, respectively[1].
Herbacitrin (2.1 μM; 5 days) significantly inhibits HIV-1 replication in HIV-1-infected MT-4 and MT-2 human T-cell lines, as measured by reduced p24 core protein levels[1].
Herbacitrin (2.15-21.5 μM) significantly inhibits recombinant HIV-1 reverse transcriptase activity at 21.5 μM, but not at 2.15 μM[1].
Herbacitrin (2.15-21.5 μM) significantly inhibits recombinant HIV-1 integrase activity at both 2.15 μM and 21.5 μM, with greater potency at the higher concentration[1].
Herbacitrin (10-50 μM; 6 days) dose-dependently inhibits the growth of normal Balb/c-derived SFME cells, shows biphasic stimulatory/inhibitory effects on ras-transformed Balb/c-derived SFME cells, and exerts only weak growth inhibition on ras/myc-transformed Balb/c-derived SFME cells, with complete inhibition of normal cells occurring at 40-50 μM[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HIV-1-infected MT-4, MT-2 human T-cell lines
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Concentration:2.1 μM
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Incubation Time:5 days
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Result:Reduced HIV-1 replication in both MT-4 and MT-2 cells, as shown by decreased p24 core protein levels, and exhibited statistically significant differences compared with the negative control.
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Cell Line:normal serum-free mouse embryo (SFME) cells, ras-transformed SFME cells, ras/myc-transformed SFME cells (all derived from Balb/c mouse embryonic cells)
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Concentration:10 μM, 20 μM, 30 μM, 40 μM, 50 μM
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Incubation Time:6 days
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Result:Markedly inhibited normal SFME cell growth dose-dependently, induced biphasic growth regulation in ras-transformed SFME cells, and weakly suppressed ras/myc-transformed SFME cell proliferation.
Chemical Information
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CAS No. 35815-07-7
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Molecular Weight 464.38
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Formula C21H20O12
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SMILES
OC1=C2C(C(C(O)=C(C3=CC=C(C=C3)O)O2)=O)=C(C=C1O[C@@H]4O[C@@H]([C@H]([C@@H]([C@H]4O)O)O)CO)O
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Research Protocol for Infectious Diseases
Infectious-disease experiments test how pathogens interact with host barriers, innate immune receptors, inflammatory signaling, pathogen replication, and tissue injury; pattern-recognition receptors such as TLRs, RIG-I-like receptors, NOD-like receptors, and inflammasomes detect microbial molecules and activate NF-κB, interferon, and cytokine responses. The central hypothesis is that infection severity reflects the balance between pathogen burden and host response: protective inflammation restricts pathogen growth, whereas excessive or mislocalized inflammation contributes to tissue damage and disease phenotype. Unresolved questions include which host pathways are protective versus pathogenic, why some infection models fail to translate to human disease, and which combined readouts best predict clinically relevant infection outcomes.
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Herbacitrin Related Classifications
HY-N15438 Related Classifications
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