m7GpppGpG
m7GpppGpG is an unmodified trinucleotide cap analog with enhancer activity. When incorporated into mRNA, it effectively enhances translational efficiency, stability, and protein expression levels of the mRNA. m7GpppGpG can serve as a standard cap analog for in vitro mRNA synthesis to support antigen generation and presentation by dendritic cells, although its expression efficiency in dendritic cells is 20-fold lower than that of the m7GpppApG cap.
For research use only. We do not sell to patients.
- CAS No.: 133608-76-1
- Formula: C31H41N15O25P4
- Molecular Weight:1147.64
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
All DNA/RNA Synthesis Isoforms
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Biological Activity
m7GpppGpG (10 mM; 2 total hours at 37°C) generates the functional m7GpppGpG cap structure alongside non-functional reversed-capped molecules during in vitro mRNA synthesis, with overall capping efficiency of ~90% under typical conditions[1].
mRNA capped with m7GpppGpG exhibits 20-fold lower translation efficiency in JAWSII murine dendritic cells relative to mRNA capped with m7GpppApG, independent of eIF4E affinity and decapping enzyme resistance[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 133608-76-1
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Molecular Weight 1147.64
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Formula C31H41N15O25P4
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SMILES
O=P(O)(OC[C@@H]1[C@@H](OP(O)(OC[C@H]2O[C@@H](N3C(NC(N)=NC4=O)=C4N=C3)[C@H](O)[C@@H]2O)=O)[C@@H](O)[C@H](N5C(NC(N)=NC6=O)=C6N=C5)O1)OP(OP(OC[C@H]7O[C@@H](N8C(NC(N)=N9)=C([N+](C)=C8)C9=O)[C@H](O)[C@@H]7O)([O-])=O)(O)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Grudzien-Nogalska E, et al. Synthetic mRNAs with superior translation and stability properties. Methods in molecular biology (Clifton, N.J.). 2013;969:55-72. [Content Brief]
[2]. Uchida S, et al. Nanomedicine-Based Approaches for mRNA Delivery. Molecular pharmaceutics. 2020 Oct 05;17(10):3654-3684. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
m7GpppGpG Related Classifications
HY-145976 Related Classifications
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