PQ32
PQ32 is an antitumor agent that targets c-MYC Pu27 and KRAS G-quadruplexes. PQ32 inhibits tumor cell proliferation, arrests the cell cycle at the G2 phase, and induces apoptosis. PQ32 inhibits the expression of c-MYC and KRAS genes. PQ32 can inhibit tumor growth in mice and is used in the study of lymphoma, breast cancer, lung cancer, hepatocellular carcinoma, colorectal cancer, and other cancers.
For research use only. We do not sell to patients.
- Formula: C27H21N3O6
- Molecular Weight:483.47
-
Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Chemical Information
- Protocols
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Description
IC50 & Target
[1]|
K-RAS |
In Vitro
PQ32 shows anti-tumor activity against RAJI, CA46, MDA-MB-231, A549, HepG2, HT29, HEK-293T cells, with IC50s of 1.75, 2.68, 1.07, 3.48, 2.72, 1.50, 4.09 μM, respectively[1].
PQ32 (1.5 μM, 24 h) induces cell cycle arrest and apoptosis in MDA-MB-231 cells[1].
PQ32 (1.5-3 μM, 24-72 h) inhibits c-MYC and KRAS gene and protein expression in MDA-MB-231 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Cell Line:MDA-MB-231
-
Concentration:1.5 μM
-
Incubation Time:24 h
-
Result:Arrested the cells in the G2 phase (increasing from 4.01% to 27.01%).
-
Cell Line:MDA-MB-231
-
Concentration:1.5 μM
-
Incubation Time:24 h
-
Result:Induced cell apoptosis (46.84%).
-
Cell Line:MDA-MB-231
-
Concentration:1.5 μM, 3 μM
-
Incubation Time:24 h, 48 h, 72 h
-
Result:Inhibited c-MYC and KRAS gene expression.
-
Cell Line:MDA-MB-231
-
Concentration:1.5 μM, 3 μM
-
Incubation Time:24 h, 48 h, 72 h
-
Result:Inhibited c-MYC and KRAS protein expression.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
-
Animal Model:HT29 cancer xenografts (5 × 106 cells, s.c.) female nude mice (BALB/c, 4-weeks old, 14-17 g) [1]
-
Dosage:15 mg/kg
-
Administration:intratumoral injection, every other day, 21 days
-
Result:Resulted in a significant reduction in tumor weight with tumor growth inhibition of 59%, displayed time-dependent inhibition of tumor growth.
- RNA extraction experimental
- Somatic Cell Culture
- Liver Cancer Modeling
- Breast Cancer Modeling
- Detection of Apoptosis
- TUNEL staining for apoptotic DNA fragmentation
- Flow cytometric DNA-content cell-cycle staining
- Apoptosis Solutions
- MTT Cell Proliferation Assay
- Annexin V plus membrane-impermeant dye apoptosis staining
- BrdU Incorporation Assay
- CFSE Dye Dilution Proliferation Assay
- Dye-dilution cell tracking and proliferation staining
- Patient-Derived Orthotopic Xenograft (PDOX)
- CCK-8/WST-8 Cell Proliferation Assay
- Protocol for Cell Cycle
- Cell Counting-Based Growth Curve Assay
- Colony Formation (Clonogenic) Assay
- Cell Viability Determination by MTT Colorimetric Assay
- EdU Incorporation Assay (Click Chemistry-Based DNA Synthesis Measurement)
- Ki-67 Immunostaining Proliferation Assay
- PCNA Immunodetection Proliferation Assay
- Protocol for Cell Counting and Cell Density Analysis
- Patient-Derived Xenograft (PDX)
- Subcutaneous Cell-Line-Derived Xenograft
- Orthotopic Cell-Line Xenograft
Chemical Information
-
Molecular Weight 483.47
-
Formula C27H21N3O6
-
SMILES
COC(C=CC(CN1C=C2C(C3=CC4=C(C=C3)OCO4)=NN=C2C5=C1C=C6OCOC6=C5)=C7)=C7OC
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
PQ32 Related Classifications
HY-175265 Related Classifications
Powered by Bioz