Cy 5 hydrazide
Based on 1 Customer Validation
Cy 5 hydrazide (Cyanine5 hydrazide) is a fluorescent dye for aldehydes and ketones. Cy 5 hydrazide can be used for labeling proteins.
For research use only. We do not sell to patients.
- Purity: 95.00%
- CAS No.: 1427705-31-4
- Formula: C32H42Cl2N4O
- Molecular Weight:569.61
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Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
Cell viability assay[1]:
1. Add Cy 5 hydrazide to sample at 25°C for 1 h to label sample.
2. Remove salts and unbound dye by the trichloroacetic acid (TCA) esodium deoxycholate (DOC) or acetone method.
3. Measure fluorescence intensity (Ex=640 nm, Em=660 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
670
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Chemical Information
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CAS No. 1427705-31-4
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Appearance Solid
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Molecular Weight 569.61
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Formula C32H42Cl2N4O
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Color Dark purple to black
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SMILES
O=C(NN)CCCCC[N+](C1=CC=CC=C1C(C)2C)=C2/C=C/C=C/C=C(C3(C)C)\N(C4=CC=CC=C34)C.[Cl-].Cl
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Synonyms
Cyanine5 hydrazide
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 50 mg/mL (87.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7556 mL | 8.7779 mL | 17.5559 mL | 43.8897 mL |
| 5 mM | 0.3511 mL | 1.7556 mL | 3.5112 mL | 8.7779 mL | |
| 10 mM | 0.1756 mL | 0.8778 mL | 1.7556 mL | 4.3890 mL | |
| 15 mM | 0.1170 mL | 0.5852 mL | 1.1704 mL | 2.9260 mL | |
| 20 mM | 0.0878 mL | 0.4389 mL | 0.8778 mL | 2.1945 mL | |
| 25 mM | 0.0702 mL | 0.3511 mL | 0.7022 mL | 1.7556 mL | |
| 30 mM | 0.0585 mL | 0.2926 mL | 0.5852 mL | 1.4630 mL | |
| 40 mM | 0.0439 mL | 0.2194 mL | 0.4389 mL | 1.0972 mL | |
| 50 mM | 0.0351 mL | 0.1756 mL | 0.3511 mL | 0.8778 mL | |
| 60 mM | 0.0293 mL | 0.1463 mL | 0.2926 mL | 0.7315 mL | |
| 80 mM | 0.0219 mL | 0.1097 mL | 0.2194 mL | 0.5486 mL |
Cy 5 hydrazide Related Classifications
HY-137100 Related Classifications
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