DNS-pE
DNS-pE is a 3-phosphoglycerate dehydrogenase (PHGDH) inhibitor with a Ki of 7.4 μM. DNS-pE selectively and irreversibly covalently modifies endogenous PHGDH in live mammalian cells, its vinyl sulfone moiety acts as a fluorescence quencher that becomes highly fluorescent upon covalent modification of PHGDH. DNS-pE can be used as a fluorescent dye for live-cell imaging.
For research use only. We do not sell to patients.
- CAS No.: 2196245-98-2
- Formula: C14H15NO2S
- Molecular Weight:261.34
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
DNS-pE (10 μM; 1 h) shows robust fluorescence Turn-ON activity with cysteine, achieving a 21-fold increase in fluorescence after 1 h of incubation at room temperature[1].
DNS-pE (1-50 μM; 3 h) concentration-dependently compets with a clickable IA probe for binding to endogenous PHGDH in live MCF-7 cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Cell Line:MCF-7 cells
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Concentration:1; 2; 5; 10; 20; 50 μM
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Incubation Time:3 h
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Result:Reduced 56-kDa PHGDH band fluorescence in a concentration-dependent manner.
Competitively binded with a clickable IA probe to endogenous PHGDH in live MCF-7 cells.
Chemical Information
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CAS No. 2196245-98-2
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Molecular Weight 261.34
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Formula C14H15NO2S
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SMILES
CN(C)C1=CC=C2C=C(S(=O)(C=C)=O)C=CC2=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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