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Cat. No.: HY-P1505

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C3a (70-77)

(Synonyms: Complement 3a (70-77))

1 Cited Publications

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Based on 1 publication(s) in Google Scholar

C3a (70-77) is an octapeptide corresponding to the COOH terminus of C3a, exhibits the specificity and 1 to 2% biologic activities of C3a.

For research use only. We do not sell to patients.

CAS No.: 63555-63-5
Formula: C₃₅H₆₁N₁₃O₁₀
Molecular Weight:823.94

Storage:

Please store the product under the recommended conditions in the Certificate of Analysis.

C3a (70-77) Chemical Structure

CAS No.: 63555-63-5

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Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Biological Activity
- Description
- IC₅₀ & Target
- In Vitro
Chemical Information
Publications (1)
Protocol
Purity & Documentation
References
Calculators
Help & FAQs
Classifications
Bioz

Biological Activity

Description

C3a (70-77) is an octapeptide corresponding to the COOH terminus of C3a, exhibits the specificity and 1 to 2% biologic activities of C3a.

IC₅₀ & Target

Complement system^[1]

In Vitro

The direct interaction of C3a(70-77) with human mononuclear leukocytes in culture results in a concentration-dependent inhibition of the generation of LIF evoked by mitogens and by the antigen SK-SD. The extent of suppression of LIF generation by C3a(70-77) is significant at concentrations of 10^-7 M or higher and exceeds 75% at 10^-6 M, irrespective of the stimulus^[1].
C3a (70-77) induces histamine release and degranulation of rat mast cells, promotes contraction of guinea pig ileal tissue, and enhances vascular permeability in human skin. C3a (70-77) selectively desensitizes ileal smooth muscle to C3a but not to human C5a, a related anaphylatoxin. Conversely, C3a-(70-77) is unable to contract ileal smooth muscle pretreated with natural C3a. This cross-desensitization indicates a specific interaction of C3a (70-77) with cellular C3a binding sites^[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Chemical Information

CAS No. 63555-63-5
Molecular Weight 823.94
Formula C₃₅H₆₁N₁₃O₁₀
Synonyms

Complement 3a (70-77)
Sequence

Ala-Ser-His-Leu-Gly-Leu-Ala-Arg
Sequence Shortening

ASHLGLAR
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

Publications (1)

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Research Square Preprint

2020 Dec.

Protocol

Kinase Assay^[1]

2×10⁵ mononuelear leukocytes in 0.2 mL of M199-HPS containing 15% (vol/vol) AB-positive serum are added to each well of microtiter plates without or with 10 μg/mL of Con A, PHA, or SK-SD and incubated at 37°C in 5% CO₂:95% air. C3a or C3a(70-77) is introduced into some suspensions at the same time as the stimulus or buffer. After 3 d for the mitogen-stimulated cultures and 5 d for the SK-SD-stimulated cultures, 1 μCi of [³H]thymidine is added to each well. After an additional 16 h at 37°C, the uptake of [³H]thymidine is analyzed by aspirating the contents of each well with a Mash II harvester that collected and ished the leukocytes on glass fiber filters. The radioactivity in each filter is quantified, and the results for quadruplicate chambers are expressed as mean cpm+SEM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Purity & Documentation

Select Batch:

Purity: 99.98%

Assay: 99.98%

ee.: 99.98%

Data Sheet (263 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Handling Instructions (2659 KB)

References

[1]. Payan DG, et al. Modulation of human lymphocyte function by C3a and C3a(70-77). J Exp Med. 1982 Sep 1;156(3):756-65. [Content Brief]

[2]. Caporale LH, et al. The active site of C3a anaphylatoxin. J Biol Chem. 1980 Nov 25;255(22):10758-63. [Content Brief]

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Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

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