eIF4E-IN-2
Based on 1 Customer Validation
eIF4E-IN-2 is a potent inhibitor of eukaryotic initiation factor 4e (eIF4e) with an IC50 of 13 nM. eIF4E-IN-2 inhibits MDA-MB-361 cells proliferation. eIF4E-IN-2 can be used for the study of breast cancer.
For research use only. We do not sell to patients.
- Purity : 98.91%
- CAS No.: 2575840-38-7
- Formula: C37H33ClF2N8O4S2
- Molecular Weight:791.29
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
Description
IC50 & Target
[1]|
eIF4 13 nM () |
In Vitro
eIF4E-IN-2 (Compound 1188) inhibits MDA-MB-361 cells proliferation with an IC50 of 46.8 nM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 2575840-38-7
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Appearance Solid
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Molecular Weight 791.29
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Formula C37H33ClF2N8O4S2
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Color Light yellow to yellow
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SMILES
N#CC1=C(N(C2CCN(C3CC(F)(C3)F)CC2)C)N=CC(N=C(C)N4CC#CC5=CC=C(C=C5C6=CC=NC7=C6SC=C7C(NS(=O)(C)=O)=O)Cl)=C1C4=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
In Vitro:
DMSO : 50 mg/mL (63.19 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Breast Cancer Modeling
Breast cancer is a heterogeneous cancer, and it has been distinguished into four subtypes: luminal A, luminal B, HER2-positive and basal-like. Molecular mutations, epigenetic alterations, hormone exposure and immune microenvironment are related to the progression of breast cancer.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
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Data Sheet (272 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
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| DMSO | 1 mM | 1.2638 mL | 6.3188 mL | 12.6376 mL | 31.5940 mL |
| 5 mM | 0.2528 mL | 1.2638 mL | 2.5275 mL | 6.3188 mL | |
| 10 mM | 0.1264 mL | 0.6319 mL | 1.2638 mL | 3.1594 mL | |
| 15 mM | 0.0843 mL | 0.4213 mL | 0.8425 mL | 2.1063 mL | |
| 20 mM | 0.0632 mL | 0.3159 mL | 0.6319 mL | 1.5797 mL | |
| 25 mM | 0.0506 mL | 0.2528 mL | 0.5055 mL | 1.2638 mL | |
| 30 mM | 0.0421 mL | 0.2106 mL | 0.4213 mL | 1.0531 mL | |
| 40 mM | 0.0316 mL | 0.1580 mL | 0.3159 mL | 0.7898 mL | |
| 50 mM | 0.0253 mL | 0.1264 mL | 0.2528 mL | 0.6319 mL | |
| 60 mM | 0.0211 mL | 0.1053 mL | 0.2106 mL | 0.5266 mL |
eIF4E-IN-2 Related Classifications
HY-145262 Related Classifications
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