X-34
Based on 1 Customer Validation
X-34 is a lipophilic and bright yellow-green fluorescent derivative of Congo red (HY-D0236). X-34 can be used to stain neuritic and diffuse plaques, neurofibrillary tangles (NFTs), neuropil threads, and cerebrovascular amyloid in the brain. X-34 can be used for research of Alzheimer’s disease.
For research use only. We do not sell to patients.
- Purity: 96.47%
- CAS No.: 215294-98-7
- Formula: C24H18O6
- Molecular Weight:402.40
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Sample preparation[1]
1.1 Paraformaldehyde-fixed tissue was cryoprotected by sequential incubation in 15% and 30% sucrose (in 0.1 M phosphate buffer), frozen, and cryosectioned.
1.2 Frozen sections were collected in Falcon 6-well plates containing
0.1 M phosphate buffer, in a 1:6 series.
1.3 Sections are stored at 4°C until assayed.
1.4 Before staining, the sections are washed for 5 min in PBS.
2. X-34 General Staining Procedure[1]
2.1 Quenched or unquenched tissue sections are taken from PBS.
2.2 Add into a solution of X-34 in 40% ethanol/60% distilled H2O
(adjusted to pH 10 by addition of 1 N NaOH) at the appropriate concentration (100 nM to 1 mM) for the appropriate
period of time (0.5-10 min).
2.3 The sections are then dipped briefly five times in tapwater before differentiation in 0.2% NaOH in 80% ethanol for 2 min.
2.4 The sections are then placed in tapwater for 10 min for microscopic observation.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
| NCT Number | Sponsor | Condition | Start Date |
Phase
|
|---|---|---|---|---|
| NCT01329991 | Plexxikon| | 2011-05 | PHASE1 |
Chemical Information
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CAS No. 215294-98-7
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Appearance Solid
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Molecular Weight 402.40
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Formula C24H18O6
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Color Light yellow to yellow
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SMILES
O=C(O)C1=C(O)C=CC(/C=C/C2=CC=C(/C=C/C3=CC(C(O)=O)=C(O)C=C3)C=C2)=C1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 100 mg/mL (248.51 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (271 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
[1]. Styren SD, et al. X-34, a fluorescent derivative of Congo red: a novel histochemical stain for Alzheimer's disease pathology. J Histochem Cytochem. 2000 Sep;48(9):1223-32. [Content Brief]
[2]. Link CD, et al. Visualization of fibrillar amyloid deposits in living, transgenic Caenorhabditis elegans animals using the sensitive amyloid dye, X-34. Neurobiol Aging. 2001 Mar-Apr;22(2):217-26. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.4851 mL | 12.4254 mL | 24.8509 mL | 62.1272 mL |
| 5 mM | 0.4970 mL | 2.4851 mL | 4.9702 mL | 12.4254 mL | |
| 10 mM | 0.2485 mL | 1.2425 mL | 2.4851 mL | 6.2127 mL | |
| 15 mM | 0.1657 mL | 0.8284 mL | 1.6567 mL | 4.1418 mL | |
| 20 mM | 0.1243 mL | 0.6213 mL | 1.2425 mL | 3.1064 mL | |
| 25 mM | 0.0994 mL | 0.4970 mL | 0.9940 mL | 2.4851 mL | |
| 30 mM | 0.0828 mL | 0.4142 mL | 0.8284 mL | 2.0709 mL | |
| 40 mM | 0.0621 mL | 0.3106 mL | 0.6213 mL | 1.5532 mL | |
| 50 mM | 0.0497 mL | 0.2485 mL | 0.4970 mL | 1.2425 mL | |
| 60 mM | 0.0414 mL | 0.2071 mL | 0.4142 mL | 1.0355 mL | |
| 80 mM | 0.0311 mL | 0.1553 mL | 0.3106 mL | 0.7766 mL | |
| 100 mM | 0.0249 mL | 0.1243 mL | 0.2485 mL | 0.6213 mL |
X-34 Related Classifications
HY-125962 Related Classifications
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