INT
Based on 3 publication(s) in Google Scholar
INT (Iodonitrotetrazolium chloride) is a biochemical reagent mainly used in the iodonitrotetrazolium chloride (INT) colorimetric method to assess the metabolic activity of cells and the activity of enzymes.
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- Pureté : 99.57%
- CAS No.: 146-68-9
- Formule: C19H13ClIN5O2
- Masse moléculaire:505.70
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Stockage:
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
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Activité biologique
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Chemical Information
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Solvant et solubilité
- Protocole
- Pureté et documentation
- Références
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Help & FAQs
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Publications Citing Use of MedChemExpress (MCE) INT
More
Activité biologique
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
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| CHO | IC50 |
49 μM
Compound: iodonitrotetrazolium chloride
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Toxicity in CHO cells
Toxicity in CHO cells
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[PMID: 18078758] |
In Vitro
INT can be used as a substrate for detection of dehydrogenase activity. Dehydrogenases are a class of enzymes that catalyze the oxidation of organic compounds by transferring hydrogen atoms to soluble INT to form red formazan, which can be quantitatively detected by colorimetry. INT acts as an electron acceptor to detect dehydrogenase activity and can be used to monitor microbial respiration, cell viability, and bacterial metabolism[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 146-68-9
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Appearance Solid
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Masse moléculaire 505.70
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Formule C19H13ClIN5O2
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Color Yellow to brown
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SMILES
IC1=CC=C(N2N=C(C3=CC=CC=C3)N=[N+]2C4=CC=C([N+]([O-])=O)C=C4)C=C1.[Cl-]
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Synonyms
Iodonitrotetrazolium chloride; p-Iodonitrotetrazolium Violet
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Livraison
Room temperature in continental US; may vary elsewhere.
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Stockage
4°C, sealed storage, away from moisture
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
Publications (3)
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Journal Impact Factor
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Most Recent
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Biosens Bioelectron
A biosensor for D-2-hydroxyglutarate in frozen sections and intraoperative assessment of IDH mutation status. [Abstract]2024 Mar 1:247:115921. PMID: 38104390 -
ACS Appl Mater Interfaces
Tetrazolium-Based Visually Indicating Bacteria Sensor for Colorimetric Detection of Point of Contamination. [Abstract]2022 Aug 24;14(33):38153-38161. PMID: 35946791 -
Mitochondrion
Sensitivity of primary mitochondrial disease fibroblasts to ferroptosis: The role of intracellular iron. [Abstract]2026 Mar:87:102112. PMID: 41581630
Solvant et solubilité
In Vitro:
DMSO : 83.33 mg/mL (164.78 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
H2O : < 0.1 mg/mL (insoluble)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (4.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.08 mg/mL (4.11 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocole
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MTT Cell Proliferation Assay
The MTT assay is a colorimetric endpoint assay for estimating viable cell number, cell growth, cytotoxicity, or cell activation in cultured mammalian cells. Living cells reduce the yellow tetrazolium salt MTT into purple/blue formazan, while dead cells do not generate the same signal; the resulting color can be quantified with a multiwell spectrophotometer. MTT reduction is commonly interpreted as a readout of metabolic activity that often correlates with viable cell number, but it should not be treated as a direct cell-counting method unless the assay is optimized for the cell type and experimental condition. Studies show that MTT reduction can involve mitochondrial and non-mitochondrial reducing systems, and formazan may accumulate in intracellular lipid droplets rather than simply marking mitochondria.
Pureté et documentation
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Fiche technique (277 KB)
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SDS (545 KB)
- English - EN (545 KB)
- Français - FR (545 KB)
- Deutsch - DE (545 KB)
- Norwegian - NO (545 KB)
- Español - ES (545 KB)
- Swedish - SV (545 KB)
- Italian - IT (545 KB)
- Korean - KR (545 KB)
- Portuguese - PT (545 KB)
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Instruction de manipulation (2659 KB)
Références
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9775 mL | 9.8873 mL | 19.7746 mL | 49.4364 mL |
| 5 mM | 0.3955 mL | 1.9775 mL | 3.9549 mL | 9.8873 mL | |
| 10 mM | 0.1977 mL | 0.9887 mL | 1.9775 mL | 4.9436 mL | |
| 15 mM | 0.1318 mL | 0.6592 mL | 1.3183 mL | 3.2958 mL | |
| 20 mM | 0.0989 mL | 0.4944 mL | 0.9887 mL | 2.4718 mL | |
| 25 mM | 0.0791 mL | 0.3955 mL | 0.7910 mL | 1.9775 mL | |
| 30 mM | 0.0659 mL | 0.3296 mL | 0.6592 mL | 1.6479 mL | |
| 40 mM | 0.0494 mL | 0.2472 mL | 0.4944 mL | 1.2359 mL | |
| 50 mM | 0.0395 mL | 0.1977 mL | 0.3955 mL | 0.9887 mL | |
| 60 mM | 0.0330 mL | 0.1648 mL | 0.3296 mL | 0.8239 mL | |
| 80 mM | 0.0247 mL | 0.1236 mL | 0.2472 mL | 0.6180 mL | |
| 100 mM | 0.0198 mL | 0.0989 mL | 0.1977 mL | 0.4944 mL |
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