IR 813 tosylate
Based on 1 Customer Validation
IR 813 tosylate is a near-infrared (NIR) fluorescent dye and can be used for visualizing regional lymph nodes in mice.
For research use only. We do not sell to patients.
- Purity: 98.52%
- CAS No.: 134127-48-3
- Formula: C47H47ClN2O3S
- Molecular Weight:755.41
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Animal: Ten-week-old female Balb/cOlaAnN mice are kept in a 12 h light/dark cycle to reduce tissue autofluorescence in the NIR region, and had access to food and water ad libitum.
2. Dose: A single dose 5.1 nmol of IR 813 dye (20 μL of 0.173 mg/mL, dissolved in a PEG-400/ethanol/water=3:2:5, v/v/v solution) is subcutaneous injected in the right anterior paw of mice.
3. Imaging: Using the Fluobeam700 NIR imaging system to perform in vivo optical imaging (739 nm excitation light (3.5 mW), 750 nm long-pass emission cutoff filter).Fluorescence intensity in the axillary lymph node (ALN) is recorded for 1 week (5 min, 1 h, 4 h, 24 h, 7 days).
4. Data analysis: Semiquantitative data is obtained from the fluorescence images using ImageJ 1.44 software.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 134127-48-3
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Appearance Solid
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Molecular Weight 755.41
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Formula C47H47ClN2O3S
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Color Brown to red
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SMILES
C[N+]1=C(/C=C/C2=C(Cl)/C(CCC2)=C/C=C3N(C)C4=C(C5=CC=CC=C5C=C4)C/3(C)C)C(C)(C)C6=C1C=CC7=CC=CC=C67.O=S(C8=CC=C(C)C=C8)([O-])=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 62.5 mg/mL (82.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (272 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Korean - KR (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.3238 mL | 6.6189 mL | 13.2378 mL | 33.0946 mL |
| 5 mM | 0.2648 mL | 1.3238 mL | 2.6476 mL | 6.6189 mL | |
| 10 mM | 0.1324 mL | 0.6619 mL | 1.3238 mL | 3.3095 mL | |
| 15 mM | 0.0883 mL | 0.4413 mL | 0.8825 mL | 2.2063 mL | |
| 20 mM | 0.0662 mL | 0.3309 mL | 0.6619 mL | 1.6547 mL | |
| 25 mM | 0.0530 mL | 0.2648 mL | 0.5295 mL | 1.3238 mL | |
| 30 mM | 0.0441 mL | 0.2206 mL | 0.4413 mL | 1.1032 mL | |
| 40 mM | 0.0331 mL | 0.1655 mL | 0.3309 mL | 0.8274 mL | |
| 50 mM | 0.0265 mL | 0.1324 mL | 0.2648 mL | 0.6619 mL | |
| 60 mM | 0.0221 mL | 0.1103 mL | 0.2206 mL | 0.5516 mL | |
| 80 mM | 0.0165 mL | 0.0827 mL | 0.1655 mL | 0.4137 mL |
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