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  4. IR 813 tosylate

IR 813 tosylate is a near-infrared (NIR) fluorescent dye (λex=815 nm, λem=840 nm) and can be used for visualizing regional lymph nodes in mice.

For research use only. We do not sell to patients.

IR 813 tosylate Chemical Structure

IR 813 tosylate Chemical Structure

CAS No. : 134127-48-3

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IR 813 tosylate Related Antibodies

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Description

IR 813 tosylate is a near-infrared (NIR) fluorescent dye (λex=815 nm, λem=840 nm) and can be used for visualizing regional lymph nodes in mice[1].

In Vitro

IR 813 (17.3 μM, 2 h) tosylate induces a 31.4% hemolysis in red blood cells[1].
IR 813 (5.9 μM, 24 h) tosylate induces 50% cell death in MRC-5 cells[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IR 813 tosylate Related Antibodies
In Vivo

IR 813 tosylate displays a maximum fluorescence intensity at 4 h postinjection together with a rapid extravasation, when being used for visualizing regional lymph nodes in mice[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Animal: Ten-week-old female Balb/cOlaAnN mice are kept in a 12 h light/dark cycle to reduce tissue autofluorescence in the NIR region, and had access to food and water ad libitum.
2. Dose: A single dose 5.1 nmol of IR 813 dye (20 μL of 0.173 mg/mL, dissolved in a PEG-400/ethanol/water=3:2:5, v/v/v solution) is subcutaneous injected in the right anterior paw of mice.
3. Imaging: Using the Fluobeam700 NIR imaging system to perform in vivo optical imaging (739 nm excitation light (3.5 mW), 750 nm long-pass emission cutoff filter).Fluorescence intensity in the axillary lymph node (ALN) is recorded for 1 week (5 min, 1 h, 4 h, 24 h, 7 days).
4. Data analysis: Semiquantitative data is obtained from the fluorescence images using ImageJ 1.44 software.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Molecular Weight

755.41

Formula

C47H47ClN2O3S
CAS No.
Appearance

Solid

Color

Light brown to brown

SMILES

C[N+]1=C(/C=C/C2=C(Cl)/C(CCC2)=C/C=C3N(C)C4=C(C5=CC=CC=C5C=C4)C/3(C)C)C(C)(C)C6=C1C=CC7=CC=CC=C67.O=S(C8=CC=C(C)C=C8)([O-])=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

4°C, sealed storage, away from moisture and light

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)

Solvent & Solubility
In Vitro: 

DMSO : 62.5 mg/mL (82.74 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

Preparing
Stock Solutions
Concentration Solvent Mass 1 mg 5 mg 10 mg
1 mM 1.3238 mL 6.6189 mL 13.2378 mL
5 mM 0.2648 mL 1.3238 mL 2.6476 mL
View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

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Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

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Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

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In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:

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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Calculation results:
Working solution concentration: mg/mL
Purity & Documentation
References

Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Optional Solvent Concentration Solvent Mass 1 mg 5 mg 10 mg 25 mg
DMSO 1 mM 1.3238 mL 6.6189 mL 13.2378 mL 33.0946 mL
5 mM 0.2648 mL 1.3238 mL 2.6476 mL 6.6189 mL
10 mM 0.1324 mL 0.6619 mL 1.3238 mL 3.3095 mL
15 mM 0.0883 mL 0.4413 mL 0.8825 mL 2.2063 mL
20 mM 0.0662 mL 0.3309 mL 0.6619 mL 1.6547 mL
25 mM 0.0530 mL 0.2648 mL 0.5295 mL 1.3238 mL
30 mM 0.0441 mL 0.2206 mL 0.4413 mL 1.1032 mL
40 mM 0.0331 mL 0.1655 mL 0.3309 mL 0.8274 mL
50 mM 0.0265 mL 0.1324 mL 0.2648 mL 0.6619 mL
60 mM 0.0221 mL 0.1103 mL 0.2206 mL 0.5516 mL
80 mM 0.0165 mL 0.0827 mL 0.1655 mL 0.4137 mL
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IR 813 tosylate Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IR 813134127-48-3IR813IR-813Fluorescent DyeDyenear-IR dyeNIRlymph nodesInhibitorinhibitorinhibit

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