1. Dye Reagents
  2. IR 813 perchlorate

IR 813 perchlorate 

Cat. No.: HY-D1535
Handling Instructions

IR 813 perchlorate is a near-infrared (NIR) fluorescent dye (Ex=815 nm, Em=840 nm) and can be used for visualizing regional lymph nodes in mice.

For research use only. We do not sell to patients.

IR 813 perchlorate Chemical Structure

IR 813 perchlorate Chemical Structure

CAS No. : 201024-57-9

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Description

IR 813 perchlorate is a near-infrared (NIR) fluorescent dye (Ex=815 nm, Em=840 nm) and can be used for visualizing regional lymph nodes in mice[1].

In Vitro

IR 813 (17.3 μM, 2 h) induces a 31.4% hemolysis in red blood cells[1].
IR 813 (5.9 μM, 24 h) induces 50% cell death in MRC-5 cells[1].

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

In Vivo

IR 813 perchlorate displays a maximum fluorescence intensity at 4 h postinjection together with a rapid extravasation, when being used for visualizing regional lymph nodes in mice[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)[1].
1. Animal: Ten-week-old female Balb/cOlaAnN mice are kept in a 12 h light/dark cycle to reduce tissue autofluorescence in the NIR region, and had access to food and water ad libitum.
2. Dose: A single dose 5.1 nmol of IR 813 dye (20 μL of 0.173 mg/mL, dissolved in a PEG-400/ethanol/water=3:2:5, v/v/v solution) is subcutaneous injected in the right anterior paw of mice.
3. Imaging: Using the Fluobeam700 NIR imaging system to perform in vivo optical imaging (739 nm excitation light (3.5 mW), 750 nm long-pass emission cutoff filter).Fluorescence intensity in the axillary lymph node (ALN) is recorded for 1 week (5 min, 1 h, 4 h, 24 h, 7 days).
4. Data analysis: Semiquantitative data is obtained from the fluorescence images using ImageJ 1.44 software.

MCE has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

683.66

Formula

C40H40Cl2N2O4

CAS No.
SMILES

O=Cl(=O)([O-])=O.ClC1=C(/C=C/C2=[N+](C)C3=C(C2(C)C)C4=C(C=CC=C4)C=C3)CCC/C1=C\C=C5N(C)C6=C(C\5(C)C)C7=C(C=CC=C7)C=C6

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

Please store the product under the recommended conditions in the Certificate of Analysis.

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IR 813 perchlorate
Cat. No.:
HY-D1535
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