IR 813 perchlorate 

IR 813 perchlorate is a near-infrared (NIR) fluorescent dye (Ex=815 nm, Em=840 nm) and can be used for visualizing regional lymph nodes in mice^[1].

IR 813 (17.3 μM, 2 h) induces a 31.4% hemolysis in red blood cells^[1].
IR 813 (5.9 μM, 24 h) induces 50% cell death in MRC-5 cells^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

IR 813 perchlorate displays a maximum fluorescence intensity at 4 h postinjection together with a rapid extravasation, when being used for visualizing regional lymph nodes in mice^[1].
Guidelines (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs)^[1].
1. Animal: Ten-week-old female Balb/cOlaAnN mice are kept in a 12 h light/dark cycle to reduce tissue autofluorescence in the NIR region, and had access to food and water ad libitum.
2. Dose: A single dose 5.1 nmol of IR 813 dye (20 μL of 0.173 mg/mL, dissolved in a PEG-400/ethanol/water=3:2:5, v/v/v solution) is subcutaneous injected in the right anterior paw of mice.
3. Imaging: Using the Fluobeam700 NIR imaging system to perform in vivo optical imaging (739 nm excitation light (3.5 mW), 750 nm long-pass emission cutoff filter).Fluorescence intensity in the axillary lymph node (ALN) is recorded for 1 week (5 min, 1 h, 4 h, 24 h, 7 days).
4. Data analysis: Semiquantitative data is obtained from the fluorescence images using ImageJ 1.44 software.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

683.66

C₄₀H₄₀Cl₂N₂O₄

201024-57-9

813

780

O=Cl(=O)([O-])=O.ClC1=C(/C=C/C2=[N+](C)C3=C(C2(C)C)C4=C(C=CC=C4)C=C3)CCC/C1=C\C=C5N(C)C6=C(C\5(C)C)C7=C(C=CC=C7)C=C6

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Marion Hell, et al. Surface chemistry architecture of silica nanoparticles determine the efficiency of in vivo fluorescence lymph node mapping. ACS Nano. 2013 Oct 22;7(10):8645-57.  [Content Brief]