IRBM-Z-1

Cat. No.: HY-181990 Purity: 98.61%: Data Sheet
COA

SDS
Handling Instructions
FAQs
Technical Support

IRBM-Z-1 is a non-competitive inhibitor of Zika virus (ZIKV) NS2B-NS3 protease, with an IC₅₀ of 1.8 μM. IRBM-Z-1 also exhibits inhibitory activity against the NS2B-NS3 proteases of T156I-mutated dengue virus 2 (DENV2) and West Nile virus (WNV), with IC₅₀ values of 3.9 μM and 4.7 μM, respectively. IRBM-Z-1 inhibits ZIKV replication, attenuates virus-induced cytopathic effects. IRBM-Z-1 is applicable to research related to ZIKV infection.

For research use only. We do not sell to patients.

IRBM-Z-1 Chemical Structure

CAS No. : 67629-86-1

Size	Stock	Quantity
Solid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	In-stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	In-stock	Estimated Time of Arrival: December 31
Solid
5 mg	In-stock	Estimated Time of Arrival: December 31
10 mg	In-stock	Estimated Time of Arrival: December 31
25 mg	In-stock	Estimated Time of Arrival: December 31
50 mg	In-stock	Estimated Time of Arrival: December 31
100 mg	In-stock	Estimated Time of Arrival: December 31
200 mg	Get quote
500 mg	Get quote

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This product is a controlled substance and not for sale in your territory.

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Description

In Vitro

IRBM-Z-1 (72 h) inhibits the replication of ZIKV replicons in stable Vero cells, with an EC₅₀ of 6.0 μM, and shows no cytotoxicity at concentrations up to 32 μM^[1].
IRBM-Z-1 (6 days) inhibits the ZIKV-induced cytopathic effect in Vero cells, with an EC₅₀ of 6.25 μM^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Molecular Weight

390.36

Formula

C₁₉H₁₇F₃N₄O₂

CAS No.

67629-86-1

Appearance

Solid

Color

White to off-white

SMILES

CC([N+]1=NOC([N-]C(NC2=CC=CC(C(F)(F)F)=C2)=O)=C1)CC3=CC=CC=C3

Shipping

Room temperature in continental US; may vary elsewhere.

Storage

4°C, sealed storage, away from moisture

*In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture)

Solvent & Solubility

In Vitro:

DMSO : ≥ 100 mg/mL (256.17 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	2.5617 mL	12.8087 mL	25.6174 mL
5 mM	0.5123 mL	2.5617 mL	5.1235 mL
10 mM	0.2562 mL	1.2809 mL	2.5617 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

Molarity Calculator
Dilution Calculator

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

Purity & Documentation

Purity: 98.61%

Select Batch:

Data Sheet (275 KB) SDS (252 KB)

COA (253 KB) HNMR (289 KB) LCMS (101 KB)

Handling Instructions (2659 KB)

References

[1]. Ontoria JM, et al. An allosteric inhibitor of the Zika virus NS2B-NS3 protease with oral efficacy in mouse models. Nat Commun. 2026;17(1):1439. Published 2026 Feb 10. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

DMSO	1 mM	2.5617 mL	12.8087 mL	25.6174 mL	64.0434 mL
	5 mM	0.5123 mL	2.5617 mL	5.1235 mL	12.8087 mL
	10 mM	0.2562 mL	1.2809 mL	2.5617 mL	6.4043 mL
	15 mM	0.1708 mL	0.8539 mL	1.7078 mL	4.2696 mL
	20 mM	0.1281 mL	0.6404 mL	1.2809 mL	3.2022 mL
	25 mM	0.1025 mL	0.5123 mL	1.0247 mL	2.5617 mL
	30 mM	0.0854 mL	0.4270 mL	0.8539 mL	2.1348 mL
	40 mM	0.0640 mL	0.3202 mL	0.6404 mL	1.6011 mL
	50 mM	0.0512 mL	0.2562 mL	0.5123 mL	1.2809 mL
	60 mM	0.0427 mL	0.2135 mL	0.4270 mL	1.0674 mL
	80 mM	0.0320 mL	0.1601 mL	0.3202 mL	0.8005 mL
	100 mM	0.0256 mL	0.1281 mL	0.2562 mL	0.6404 mL

IRBM-Z-1 Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

IRBM-Z-167629-86-1Virus ProteaseFlavivirusZika virus (I156T mutant)West Nile virus NS2B-NS3 proteaseZika virus NS2B-NS3 proteaseZIKV repliconZika virus infectionT156I mutant DENV2 NS2B-NS3 proteaseZika virus replicationVero cellsDENV2 NS2B-NS3 proteasestable Vero cellsInhibitorinhibitorinhibit

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IRBM-Z-1

IRBM-Z-1 Related Antibodies

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