BC‐AZIDE
BC‐AZIDE (CLIP-AZIDE) is a CLIP-tag substrate that forms a covalent irreversible linkage with CLIP-tag proteins. When paired with conjugated fluorophores, BC‐AZIDE enables specific labeling of CLIP-tagged proteins in live and fixed cells. BC‐AZIDE can be used for research related to bioorthogonal click chemistry and intracellular orthogonal labeling.
For research use only. We do not sell to patients.
- CAS No.: 2432950-51-9
- Formula: C18H23N7O4
- Molecular Weight:401.42
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
BC-AZIDE (30 min) mediates SPAAC labeling of the nuclear envelope and actin fibers by DBCO-PEG4-5/6-TAMRA in live HeLa cells, enables SPAAC/IEDDA dual labeling of SNAP-/CLIP-tag fusion proteins in live cells with no obvious cross-reactivity, and also accomplishes two-color SPAAC/IEDDA labeling of CLIP-tag targets in fixed-permeabilized HeLa cells[2][3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2432950-51-9
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Molecular Weight 401.42
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Formula C18H23N7O4
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SMILES
[N-]=[N+]=NCCOCCOCC(NCC1=CC=C(C=C1)COC2=NC(N)=CC=N2)=O
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Synonyms
CLIP-AZIDE
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Gayathri V, et al. Functionalization of Bacterial Cellulose and Related Surfaces Using a Facile Coupling Reaction by Thermoresponsive Catalyst. ACS biomaterials science & engineering. 2023 Feb 13;9(2):625-641. [Content Brief]
[2]. Krishnamurthy M, et al. Improved Hydrophobicity of a Bacterial Cellulose Surface: Click Chemistry in Action. ACS biomaterials science & engineering. 2020 Feb 10;6(2):879-888. [Content Brief]
[3]. Macias-Contreras M, et al. SNAP/CLIP-Tags and Strain-Promoted Azide-Alkyne Cycloaddition (SPAAC)/Inverse Electron Demand Diels-Alder (IEDDA) for Intracellular Orthogonal/Bioorthogonal Labeling. Bioconjugate chemistry. 2020 May 20;31(5):1370-1381. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
BC‐AZIDE Related Classifications
HY-D2955 Related Classifications
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