Janelia Fluor® 526, SE
Janelia Fluor 526, SE (JF526,SE) is a fluorogenic yellow fluorescent dye that contains NHS ester group. JF526 is a versatile scaffold for fluorogenic ligands, including labels for genetically encoded self-labeling protein tags and stains for endogenous structures. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute (Ex/Em = 526/550 nm).
For research use only. We do not sell to patients.
- Formula: C31H19F6N3O7
- Molecular Weight:659.49
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
Biological Activity
Janelia Fluor fluorescent dyes do not inherently possess HaloTag-binding activity; however, reactive groups such as NHS esters serve as reaction sites for covalent coupling-via chemical synthesis-to an amino-functionalized HaloTag chloroalkane ligand scaffold, thereby yielding a fluorescent HaloTag ligand.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
526
488
Chemical Information
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Molecular Weight 659.49
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Formula C31H19F6N3O7
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SMILES
FC(F)(C/1)C[N+]1=C2C(F)=CC3=C(C4=CC(C(ON5C(CCC5=O)=O)=O)=CC=C4C([O-])=O)C6=CC(F)=C(N7CC(F)(C7)F)C=C6OC3=C\2
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Synonyms
JF526, SE; JF526, NHS
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)