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Cat. No.: HY-131025

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Janelia Fluor® 585, SE

(Synonyms: JF585, SE; JF585, NHS)

Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. Janelia Fluor 585, SE can be used immediately for structured illumination (SIM) and stimulated emission depletion (STED) imaging and could be converted to photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

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CAS No.: 1811539-88-4
Formule: C₃₄H₂₇F₄N₃O₆
Masse moléculaire:649.59

Stockage:

Please store the product under the recommended conditions in the Certificate of Analysis.

Data Sheet (273 KB)

Janelia Fluor® 585, SE Chemical Structure

CAS No.: 1811539-88-4

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Use of MCE Products

Fluorescent Dye

Strong Fluorescence Signal / Multi-Disciplinary Applications / High Fluorescence Specificity

Activité biologique
- Description
- In Vitro
- Emission (Em)
- Excitation (Ex)
Chemical Information
Pureté et documentation
Références
Calculators
Help & FAQs
Classifications
Bioz

Activité biologique

Description

Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. Janelia Fluor 585, SE can be used immediately for structured illumination (SIM) and stimulated emission depletion (STED) imaging and could be converted to photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments^[1]. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute.

In Vitro

Maximum absorption wavelength (λabs)=585 nm, maximum emission wavelength (λem)=609 nm^[1].
The JF585-SNAP-tag ligand (24) also functioned as a cellular label. The new HaloTag ligands derived from JF585 and JF635 show a high degree of chromogenicity and fluorogenicity, a critical parameter in advanced imaging experiments^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Emission (Em)

585

Excitation (Ex)

565

Chemical Information

CAS No. 1811539-88-4
Masse moléculaire 649.59
Formule C₃₄H₂₇F₄N₃O₆
SMILES

FC(C/1)(F)C[N+]1=C2C=C3C(C)(C)C4=C(C=CC(N5CC(F)(F)C5)=C4)C(C6=CC(C(ON7C(CCC7=O)=O)=O)=CC=C6C([O-])=O)=C3C=C/2
Synonyms

JF585, SE; JF585, NHS
Livraison

Room temperature in continental US; may vary elsewhere.
Stockage

Please store the product under the recommended conditions in the Certificate of Analysis.

Pureté et documentation

Select Batch:

Pureté: 99.98%

Assay: 99.98%

ee.: 99.98%

Fiche technique (273 KB)
COA (2659 KB)
Elemental Analysis Report (2659 KB)
HNMR (2659 KB)
CNMR (2659 KB)
RP-HPLC (2659 KB)
NP-HPLC (2659 KB)
MS (2659 KB)
LCMS (2659 KB)
IR (2659 KB)
GC (2659 KB)
OR (2659 KB)
SDS-PAGE (2659 KB)
SEC-HPLC (2659 KB)
Other Report (2659 KB)
Instruction de manipulation (2659 KB)

Références

[1]. Grimm JB, Muthusamy AK, Liang Y, et al. A general method to fine-tune fluorophores for live-cell and in vivo imaging. Nat Methods. 2017;14(10):987-994. doi:10.1038/nmeth.4403 [Content Brief]

Calculators

Calculateur de molarité

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass Mass

Concentration Concentration

Volume Volume

Molecular Weight Molecular Weight

Calculateur de dilution

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

Concentration _(start) Concentration _(start)

Volume _(start) Volume _(start)

Concentration _(final) Concentration _(final)

Volume _(final) Volume _(final)

Help & FAQs

How should lyophilized recombinant proteins be reconstituted and stored?

1. Before opening the cap, centrifuge the vial at 13000 rpm for 20-30 seconds. This step will ensure that any lyophilized powder that may have adhered to the cap or walls is collected at the bottom of the vial, minimizing the risk of product loss.

Keywords

MOQ

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100 mg

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