Janelia Fluor® 585, SE
Based on 1 Customer Validation
Janelia Fluor 585, SE (JF585, SE) is an orange fluorescent dye containing an NHS ester that can be conjugated with primary amine groups. Janelia Fluor 585, SE can be used immediately for structured illumination (SIM) and stimulated emission depletion (STED) imaging and could be converted to photoactivatable derivative for single-molecule localization microscopy (SMLM) experiments. Janelia Fluor fluorescent dyes can be chemically conjugated to the specific HaloTag substrate (a chloroalkane ligand) to form a fluorescent ligand, rather than binding directly to the HaloTag protein itself. Janelia Fluor products are licensed under U.S. Pat. Nos. 9,933,417, 10,018,624 and 10,161,932 and other patents from Howard Hughes Medical Institute (Ex/Em = 585/609 nm).
For research use only. We do not sell to patients.
- Purity: 99.31%
- CAS No.: 1811539-88-4
- Formula: C34H27F4N3O6
- Molecular Weight:649.59
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Storage:
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Janelia Fluor fluorescent dyes do not inherently possess HaloTag-binding activity; however, reactive groups such as NHS esters serve as reaction sites for covalent coupling-via chemical synthesis-to an amino-functionalized HaloTag chloroalkane ligand scaffold, thereby yielding a fluorescent HaloTag ligand.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
585
565
Chemical Information
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CAS No. 1811539-88-4
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Appearance Solid
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Molecular Weight 649.59
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Formula C34H27F4N3O6
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Color Light green to green
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SMILES
FC(C/1)(F)C[N+]1=C2C=C3C(C)(C)C4=C(C=CC(N5CC(F)(F)C5)=C4)C(C6=CC(C(ON7C(CCC7=O)=O)=O)=CC=C6C([O-])=O)=C3C=C/2
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Synonyms
JF585, SE; JF585, NHS
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : ≥ 100 mg/mL (153.94 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (278 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.5394 mL | 7.6972 mL | 15.3943 mL | 38.4858 mL |
| 5 mM | 0.3079 mL | 1.5394 mL | 3.0789 mL | 7.6972 mL | |
| 10 mM | 0.1539 mL | 0.7697 mL | 1.5394 mL | 3.8486 mL | |
| 15 mM | 0.1026 mL | 0.5131 mL | 1.0263 mL | 2.5657 mL | |
| 20 mM | 0.0770 mL | 0.3849 mL | 0.7697 mL | 1.9243 mL | |
| 25 mM | 0.0616 mL | 0.3079 mL | 0.6158 mL | 1.5394 mL | |
| 30 mM | 0.0513 mL | 0.2566 mL | 0.5131 mL | 1.2829 mL | |
| 40 mM | 0.0385 mL | 0.1924 mL | 0.3849 mL | 0.9621 mL | |
| 50 mM | 0.0308 mL | 0.1539 mL | 0.3079 mL | 0.7697 mL | |
| 60 mM | 0.0257 mL | 0.1283 mL | 0.2566 mL | 0.6414 mL | |
| 80 mM | 0.0192 mL | 0.0962 mL | 0.1924 mL | 0.4811 mL | |
| 100 mM | 0.0154 mL | 0.0770 mL | 0.1539 mL | 0.3849 mL |
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