2. Apoptosis
  3. MDM-2/p53 Apoptosis
  4. JN122

JN122, a spiroindoline-containing molecule, is a MDM2 inhibitor. JN122 Inhibits MDM2/p53 protein–protein interaction and exerts robust in vivo antitumor efficacy. JN122 has antiproliferative activity in HCT-116 cells and HEK-293 cells with IC50 values of 39.6 nM and 4.28μM, respectively. JN122 can promote activation of p53 and its target genes, inhibited cell cycle progression, and induced cell apoptosis.

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JN122

JN122 Chemical Structure

CAS No. : 3035577-19-3

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JN122 Related Antibodies

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Description

JN122, a spiroindoline-containing molecule, is a MDM2 inhibitor. JN122 Inhibits MDM2/p53 protein–protein interaction and exerts robust in vivo antitumor efficacy. JN122 has antiproliferative activity in HCT-116 cells and HEK-293 cells with IC50 values of 39.6 nM and 4.28μM, respectively. JN122 can promote activation of p53 and its target genes, inhibited cell cycle progression, and induced cell apoptosis[1].

IC50 & Target

IC50: 39.6 nM (HCT-116 cells); 4.28μM (HEK-293 cells)[1].
Cellular Effect
Cell Line Type Value Description References
A549 IC50
2.9 nM
Compound: (-)60; JN122
Growth inhibition of human A549 cells harboring p53 incubated for 4 days by CCK-8 assay
Growth inhibition of human A549 cells harboring p53 incubated for 4 days by CCK-8 assay
[PMID: 38062557]
HCT-116 IC50
13.6 nM
Compound: XVI; JN122
Antiproliferative activity against human HCT-116 cells incubated for 4 days by CCK8 assay
Antiproliferative activity against human HCT-116 cells incubated for 4 days by CCK8 assay
[PMID: 38581730]
HCT-116 IC50
< 1 nM
Compound: (-)60; JN122
Antiproliferative activity against human HCT-116 cells harboring p53 assessed inhibition of cell growth incubated for 4 days by celltiter-glo assay
Antiproliferative activity against human HCT-116 cells harboring p53 assessed inhibition of cell growth incubated for 4 days by celltiter-glo assay
[PMID: 38062557]
HCT-116 IC50
< 1 nM
Compound: 60; YN55
Antiproliferative activity against human HCT-116 cells harboring p53 assessed inhibition of cell growth incubated for 4 days by celltiter-glo assay
Antiproliferative activity against human HCT-116 cells harboring p53 assessed inhibition of cell growth incubated for 4 days by celltiter-glo assay
[PMID: 38062557]
HEK293 IC50
4.28 μM
Compound: (-)60; JN122
Antiproliferative activity against HEK293 cells harboring p53 assessed inhibition of cell growth incubated for 4 days by CCK-8 assay
Antiproliferative activity against HEK293 cells harboring p53 assessed inhibition of cell growth incubated for 4 days by CCK-8 assay
[PMID: 38062557]
HEK293 IC50
5.94 μM
Compound: 60; YN55
Antiproliferative activity against HEK293 cells harboring p53 assessed inhibition of cell growth incubated for 4 days by CCK-8 assay
Antiproliferative activity against HEK293 cells harboring p53 assessed inhibition of cell growth incubated for 4 days by CCK-8 assay
[PMID: 38062557]
HeLa IC50
12.24 nM
Compound: (-)60; JN122
Growth inhibition of human HeLa cells harboring p53 incubated for 4 days by CCK-8 assay
Growth inhibition of human HeLa cells harboring p53 incubated for 4 days by CCK-8 assay
[PMID: 38062557]
HepG2 IC50
0.32 nM
Compound: (-)60; JN122
Growth inhibition of human HepG2 cells harboring p53 incubated for 4 days by celltiter glo assay
Growth inhibition of human HepG2 cells harboring p53 incubated for 4 days by celltiter glo assay
[PMID: 38062557]
MOLM-13 IC50
0.2 nM
Compound: XVI; JN122
Antiproliferative activity against human MOLM-13 cells incubated for 2 days by CCK8 assay
Antiproliferative activity against human MOLM-13 cells incubated for 2 days by CCK8 assay
[PMID: 38581730]
MOLM-13 IC50
49.9 nM
Compound: (-)60; JN122
Growth inhibition of human MOLM-13 cells incubated for 2 days by CCK-8 assay
Growth inhibition of human MOLM-13 cells incubated for 2 days by CCK-8 assay
[PMID: 38062557]
MSTO-211H IC50
9.48 nM
Compound: (-)60; JN122
Growth inhibition of human MSTO-211H cells harboring p53 incubated for 4 days by CCK-8 assay
Growth inhibition of human MSTO-211H cells harboring p53 incubated for 4 days by CCK-8 assay
[PMID: 38062557]
NCI-H460 IC50
0.26 nM
Compound: (-)60; JN122
Growth inhibition of human NCI-H460 cells harboring p53 incubated for 4 days by CCK-8 assay
Growth inhibition of human NCI-H460 cells harboring p53 incubated for 4 days by CCK-8 assay
[PMID: 38062557]
RKO IC50
124 nM
Compound: 60; YN55
Antiproliferative activity against human RKO cells harboring p53 assessed inhibition of cell growth incubated for 4 days by CCK-8 assay
Antiproliferative activity against human RKO cells harboring p53 assessed inhibition of cell growth incubated for 4 days by CCK-8 assay
[PMID: 38062557]
RKO IC50
43.2 nM
Compound: (-)60; JN122
Antiproliferative activity against human RKO cells harboring p53 assessed inhibition of cell growth incubated for 4 days by CCK-8 assay
Antiproliferative activity against human RKO cells harboring p53 assessed inhibition of cell growth incubated for 4 days by CCK-8 assay
[PMID: 38062557]
SW480 IC50
7.1 nM
Compound: (-)60; JN122
Growth inhibition of human SW480 cells incubated for 4 days by CCK-8 assay
Growth inhibition of human SW480 cells incubated for 4 days by CCK-8 assay
[PMID: 38062557]
U2OS IC50
10.8 nM
Compound: (-)60; JN122
Antiproliferative activity against human U2OS cells harboring p53 assessed inhibition of cell growth incubated for 4 days by celltiter-glo assay
Antiproliferative activity against human U2OS cells harboring p53 assessed inhibition of cell growth incubated for 4 days by celltiter-glo assay
[PMID: 38062557]
U2OS IC50
67 nM
Compound: 60; YN55
Antiproliferative activity against human U2OS cells harboring p53 assessed inhibition of cell growth incubated for 4 days by celltiter-glo assay
Antiproliferative activity against human U2OS cells harboring p53 assessed inhibition of cell growth incubated for 4 days by celltiter-glo assay
[PMID: 38062557]
Molecular Weight

610.57

Formula

C33H37Cl2N3O4
CAS No.
SMILES

CCN1[C@H]([C@@H]([C@@]2([C@@H]1CC(C)(C)C)CNC3=CC(Cl)=CC=C32)C4=CC(Cl)=CC=C4)C(NC5=CC=C(C=C5OC)C(O)=O)=O
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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JN122 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JN1223035577-19-3JN 122JN-122MDM-2/p53ApoptosisMDM2MDM2/p53 protein–protein interactionHCT-116 cellsHEK-293 cellscell cycleapoptosisInhibitorinhibitorinhibit

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