JB-95 acetate
Based on 1 Customer Validation
JB-95 acetate is an Antimicrobial peptide. JB-95 acetate disrupts the folding and insertion of β-barrel outer membrane proteins, selectively disrupts the bacterial outer membrane, and triggers σE envelope stress. JB-95 acetate causes cell elongation and membrane material accumulation, but does not alter inner membrane permeability. JB-95 acetate has activity against Gram-positive and Gram-negative bacteria, especially E. coli.
For research use only. We do not sell to patients.
- Purity : 98.39%
- Formula: C92H151N35O14.xC2H4O2
- Molecular Weight:1971.41 (free base)
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Storage:
Sealed storage, away from moisture and light, under nitrogen.
Powder -80°C, 2 years , -20°C, 1 year* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
JB-95 acetate selectively disrupts the outer membrane but not the inner membrane of Escherichia coli[1].
JB-95 acetate exposure substantially depletes many β-barrel outer membrane proteins in Escherichia coli[1].
JB-95 acetate inhibits E. coli growth with an MIC of 0.25 μg/mL and shows activity against a Gram-negative bacterial panel[2].
JB-95 acetate exposure impairs OM integrity, decreases OMP abundance, and induces cell envelope stress in E. coli[2].
JB-95 acetate reduces viability of Gram-positive cells lacking an OM, consistent with a second cytoplasmic-membrane-associated mechanism[2].
JB-95 acetate in E. coli acts as an OM-targeting chemical stressor that induces the σE envelope stress response[3].
JB-95 acetate in E. coli selectively deforms the OM without increasing IM permeability and produces cell elongation with membrane material accumulation[3].
JB-95 acetate in E. coli interacts with β-barrel OMPs including BamA and LptD, compromises BAM-dependent OMP folding/insertion, and triggers σE[3].
JB-95 acetate is active against E. coli (MIC 0.25 μg/mL), P. aeruginosa (MIC 4 μg/mL), A. baumannii (MIC 1 μg/mL), and S. aureus (MIC 2 μg/mL), and it inhibits BamA and LptD while increasing outer membrane stress[4].
JB-95 acetate selectively disrupts the outer membrane of E. coli while leaving the inner membrane intact[5].
JB-95 acetate reduces β-OMP expression in E. coli, supporting the proposed mechanism of BAM inhibition through BamA binding[5].
JB-95 acetate inhibits E. coli ATCC 25922 with an MIC of 0.15 μg/mL, with BamA or LptD listed as possible targets[5].
JB-95 acetate selectively disrupts the outer membrane but not the inner membrane of E. coli[6].
JB-95 acetate depletes many OMPs but not BamA in E. coli[6].
JB-95 acetate is a peptide that interacts with BamA, with LptD also listed as a target, and strongly inhibits Gram-positive and Gram-negative bacteria, especially Escherichia coli, through outer membrane disruption[7].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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Appearance Solid
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Molecular Weight 1971.41 (free base)
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Formula C92H151N35O14.xC2H4O2
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Color White to off-white
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Sequence
cyclo-(Trp-Arg-Ile-Arg-Ile-{d-Arg}-Trp-Lys-Arg-Leu-Arg-Arg-{d-Pro}-Pro)
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Sequence Shortening
cyclo-(WRIRI-{d-Arg}-WKRLRR-{d-Pro}-P)
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Sealed storage, away from moisture and light, under nitrogen
Powder -80°C 2 years -20°C 1 year * In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light, under nitrogen)
Solvent & Solubility
In Vitro:
H2O : ≥ 50 mg/mL
* "≥" means soluble, but saturation unknown.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Working solution concentration: 0.22 mg/mL
This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.
Purity & Documentation
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Data Sheet (292 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
JB-95 acetate Related Classifications
HY-P5753A Related Classifications
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