Probe-Cys
Based on 1 Customer Validation
Probe-Cys is a water-soluble and selective near-infrared fluorescent probe for Cysteine (Cys) (λex= 680 nm, λem=710 nm) that is not interfered by Hcy, GSH, and HS-. Probe-Cys can react with the stimulant DTT (HY-15917) and the NEM (HY-D0843) in HepG2 cells and zebrafish for the detection of endogenous Cys. Probe-Cys can also be used for imaging Cys in Arabidopsis thaliana. Probe-Cys provides a method for cancer diagnosis and exploration of plant sulfur metabolism.
商品は「研究用試薬」です。人や動物の医療用・臨床診断用・食品用の製品ではありません。
研究用途以外に使用した場合、当社は一切の責任を負いかねます。
- 純度: 98.87%
- 分子式: C33H31NO7S
- 分子量:585.67
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保管条件:
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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生物活性
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化学情報
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溶剤 & 溶解度
- 純度とドキュメンテーション
- 参考文献
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Help & FAQs
生物活性
Probe-Cys sensitively detects Cys in cell-free systems with a linear range of 0-100 μM, a detection limit of 0.035 μM, and a strong fluorescence turn-on response at 710 nm[1].
Probe-Cys (10 μM; 1 h) specifically detects endogenous and exogenous Cys in HepG2 cells, with no interference from Hcy or GSH, and responds to modulations in cellular Cys levels via DTT and NEM treatment[1].
Probe-Cys (10 μM; 1 h) penetrates Arabidopsis thaliana root tissue to detect endogenous Cys, with fluorescence responding to DTT-mediated elevation and NEM-mediated inhibition, and reveals higher Cys content in root meristematic zones compared to elongation zones[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Zebrafish (3 days old)[1]
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Dosage:20 μM
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Administration:incubation; 1 h
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Result:Exhibited weak fluorescence with relative pixel intensity ca.
32.62% (defining the Cys-pretreated group intensity as 1.0).
Showed greatly reduced fluorescence with relative pixel intensity ca. 7.54% when zebrafish were preincubated with NEM (HY-D0843).
Showed obvious fluorescence enhancement with relative pixel intensity ca. 96.61% when zebrafish were preincubated with DTT.
Produced stronger fluorescence signals in the yolk sac compared to other areas.
化学情報
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性状 Solid
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分子量 585.67
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分子式 C33H31NO7S
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Color Brown to reddish brown
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SMILES
O=C(C1=CC=CO1)OC2=CC(OC3=C4/C=C/C(C(C)(C5=CC=CC=C56)C)=[N+]6CCCCS(=O)([O-])=O)=C(C=C3CC4)C=C2
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輸送条件
Room temperature in continental US; may vary elsewhere.
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保管条件
-20°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
溶剤 & 溶解度
DMSO : 50 mg/mL (85.37 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
濃度 (開始) × 体積 (開始) = 濃度 (終了) × 体積 (終了)
純度とドキュメンテーション
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データシート (277 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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取扱説明書 (2659 KB)
参考文献
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.7074 mL | 8.5372 mL | 17.0745 mL | 42.6862 mL |
| 5 mM | 0.3415 mL | 1.7074 mL | 3.4149 mL | 8.5372 mL | |
| 10 mM | 0.1707 mL | 0.8537 mL | 1.7074 mL | 4.2686 mL | |
| 15 mM | 0.1138 mL | 0.5691 mL | 1.1383 mL | 2.8457 mL | |
| 20 mM | 0.0854 mL | 0.4269 mL | 0.8537 mL | 2.1343 mL | |
| 25 mM | 0.0683 mL | 0.3415 mL | 0.6830 mL | 1.7074 mL | |
| 30 mM | 0.0569 mL | 0.2846 mL | 0.5691 mL | 1.4229 mL | |
| 40 mM | 0.0427 mL | 0.2134 mL | 0.4269 mL | 1.0672 mL | |
| 50 mM | 0.0341 mL | 0.1707 mL | 0.3415 mL | 0.8537 mL | |
| 60 mM | 0.0285 mL | 0.1423 mL | 0.2846 mL | 0.7114 mL | |
| 80 mM | 0.0213 mL | 0.1067 mL | 0.2134 mL | 0.5336 mL |
Probe-Cys 関連分類
HY-D2315 関連分類
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