2. Apoptosis NF-κB Metabolic Enzyme/Protease Immunology/Inflammation
  3. Transferrin Receptor Reactive Oxygen Species (ROS) Ferroptosis
  4. JST-TfR09

JST-TfR09  (Synonyms: PPMX-T003)

Cat. No.: HY-P991381 Purity: 99.59%
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JST‑TfR09 (PPMX‑T003) is a human monoclonal antibody (mAb) targeting transferrin receptor 1 (TfR1/CD71). JST‑TfR09 blocks the binding of transferrin to TfR1, inhibits TfR1 internalization, and suppresses cellular iron uptake. JST‑TfR09 triggers ferritin degradation via activating the autolysosomal system, promotes ROS production and lipid peroxidation, and ultimately induces ferroptosis. JST‑TfR09 exhibits cytotoxicity toward human erythroblasts differentiated from hematopoietic stem cells. JST-TfR09 can be used in leukemia research. Recommended isotype control: Human IgG1 lambda, Isotype Control (HY-P99992).

For research use only. We do not sell to patients.

JST-TfR09

JST-TfR09 Chemical Structure

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JST-TfR09 Related Antibodies

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Description

JST‑TfR09 (PPMX‑T003) is a human monoclonal antibody (mAb) targeting transferrin receptor 1 (TfR1/CD71). JST‑TfR09 blocks the binding of transferrin to TfR1, inhibits TfR1 internalization, and suppresses cellular iron uptake. JST‑TfR09 triggers ferritin degradation via activating the autolysosomal system, promotes ROS production and lipid peroxidation, and ultimately induces ferroptosis. JST‑TfR09 exhibits cytotoxicity toward human erythroblasts differentiated from hematopoietic stem cells. JST-TfR09 can be used in leukemia research. Recommended isotype control: Human IgG1 lambda, Isotype Control (HY-P99992)[1][2].

Isotype

Human IgG1 lambda
Recommend Isotype Controls
Species Reactivity

Human
IC50 & Target

CD71
In Vitro

JST-TfR09 (10 ng/well-200 ng; 48 h) reduces viability of MT2, Su9T1, and KK1, and this cell death is inhibited by ferrostatin-1[1].
JST-TfR09 (100 ng/mL; 48 h) downregulates TFR1, FTH1, and FTL, upregulates IRP2 and NCOA4, and induces lysosome-dependent ferritin degradation and autophagy in ED, KK1, and MT2 ATLL cell lines[1].
JST-TfR09 (100 ng/mL; 48 h) increases intracellular ferrous iron, ROS, and lipid peroxidation (MDA) levels, key hallmarks of ferroptosis, in Su9T1, MT2, ST1, SO4, KK1, and ED ATLL cell lines and MOLT4, JURKAT T-ALL cell lines[1].
JST-TfR09 induces in vitro toxicity to human erythroblasts differentiated from CD34 hematopoietic stem cells at concentrations greater than 156 ng/mL[2].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

JST-TfR09 Related Antibodies

Cell Viability Assay[1]

Cell Line: adult T-cell leukemia/lymphoma (ATLL) cell lines (ED, Su9T01, S1T, KK1, KOB, ST1), HTLV-1-transformed human T-cell lines (MT2, HUT102), cutaneous T-cell lymphoma (CTCL) cell lines (HUT78, HH)
Concentration: 10 ng/well; 200 ng; 100 ng/mL
Incubation Time: 48 h
Result: Reduced the viability of MT2, Su9T1, and KK1 ATLL cell lines to 40-60% relative to controls.
Increased cell viability to almost 80% in MT2, Su9T1, and KK1 ATLL cell lines when co-treated with ferrostatin-1.

Western Blot Analysis[1]

Cell Line: ATLL cell lines (ED, KK1, MT2)
Concentration: 100 ng/mL;
25 μM Aloxistatin (HY-100229) + 50 μM Pepstatin A (HY-P0018) (pretreatment);
10 μM MG132 (HY-13259) (pretreatment)
Incubation Time: 48 h (JST-TfR09); 24 h (pretreatment prior to JST-TfR09)
Result: Decreased TFR1, FTH1, and FTL protein levels in ED and KK1 cells.
Increased IRP2 and NCOA4 protein levels in ED and KK1 cells.
Prevented JST-TfR09-induced ferritin degradation in cells pretreated with lysosomal inhibitors, while proteasomal inhibitor pretreatment did not.
Increased LC3-II expression relative to LC3-I in cells, indicating autophagy activation, which was blocked by lysosomal inhibitor co-treatment.
Gene ID

7037  [NCBI]

Accession
Application

ELISA, FACS, Functional assay
Conjugated

Unconjugated
Reconsititution

The product can be reconstituted/diluted with sterile PBS or saline.
Molecular Weight

145.79 kDa

Appearance

Liquid

Color

Colorless to light yellow

SMILES

[JST-TfR09]
Shipping

Shipping with dry ice.
Formulation

Please refer to the lot-specific COA for specific buffer information.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
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JST-TfR09 Related Classifications

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Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

JST-TfR09PPMX-T003Transferrin ReceptorReactive Oxygen Species (ROS)FerroptosisTfRCD71TfR1human erythroblastsadult T-cell leukemia/lymphomaferroptosisTransferrin Receptor 1T-ALL cell lineshematopoietic stem cellspolycythemia veraATLL cell linesInhibitorinhibitorinhibit

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