Kaempferol tetraacetate
Kaempferol tetraacetate (AF33) is a natural product. Kaempferol tetraacetate shows cytotoxicity.
For research use only. We do not sell to patients.
- CAS No.: 16274-11-6
- Formula: C23H18O10
- Molecular Weight:454.38
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
Cellular Effect
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Cell Line
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Type | Value | Description | References |
|---|---|---|---|---|
| B16 | IC50 |
40.7 μM
Compound: 16
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Cytotoxicity against mouse B16 cells assessed as cell viability after 48 hrs by MTT assay
Cytotoxicity against mouse B16 cells assessed as cell viability after 48 hrs by MTT assay
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[PMID: 25659770] |
| J774.2 | IC50 |
15.56 μM
Compound: 1a
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Cytotoxicity against BALB/c mouse J774.2 cells after 72 hrs by trypan blue assay
Cytotoxicity against BALB/c mouse J774.2 cells after 72 hrs by trypan blue assay
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[PMID: 19489596] |
In Vitro
Kaempferol tetraacetate (compound 1a) (0-100 µM; 72 h) shows cytotoxicity with IC50s of 45, 48, 37 µM for HL-60, U937, SK-MEL-1 cells, respectively[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:HL-60, U937, SK-MEL-1 cells
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Concentration:0-100 µM
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Incubation Time:72 h
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Result:Showed cytotoxicity with IC50s of 45, 48, 37 µM for HL-60, U937, SK-MEL-1 cells, respectively.
Chemical Information
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CAS No. 16274-11-6
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Molecular Weight 454.38
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Formula C23H18O10
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SMILES
CC(OC1=C2C(OC(C3=CC=C(OC(C)=O)C=C3)=C(OC(C)=O)C2=O)=CC(OC(C)=O)=C1)=O
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Synonyms
AF33
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
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Mammalian live/dead viability and cytotoxicity staining
Live/dead viability and cytotoxicity staining assays are based on the simultaneous detection of intracellular esterase activity in metabolically active (viable) cells and membrane integrity loss in non-viable cells. In commonly used dual-staining approaches, membrane-permeant fluorogenic substrates are converted by intracellular esterases into fluorescent products in live cells, while impermeant DNA-binding dyes selectively enter cells with compromised plasma membranes and label nucleic acids in dead or dying cells, enabling discrimination between viable and non-viable populations by fluorescence microscopy or flow cytometry.
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Cell Viability Determination by MTT Colorimetric Assay
The following protocol uses the MTT colorimetric assay as a classic literature-established method for assessing cell viability/metabolic activity in cultured mammalian cells. MTT[3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] is reduced by metabolically active cells to a colored formazan product; the amount of formazan is quantified spectrophotometrically and provides an indirect measure of metabolically active viable cells. Importantly, MTT reduction reflects cellular oxidoreductase/metabolic activity rather than an absolute direct count of living cells, so changes in cellular metabolism can alter the signal independently of cell number.
Purity & Documentation
References
[1]. Mei Q, et al. Selective methylation of kaempferol via benzylation and deacetylation of kaempferol acetates. Beilstein J Org Chem. 2015 Feb 25;11:288-93. [Content Brief]
[2]. Díaz JG, et al. Cytotoxic activities of flavonoid glycoside acetates from Consolida oliveriana. Planta Med. 2008 Feb;74(2):171-4. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Kaempferol tetraacetate Related Classifications
HY-N3430 Related Classifications
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