Kalopanaxsaponin I
Kalopanaxsaponin I (Septemloside I) is an oleanane-type triterpenoid that exists in the fruits of Stauntonia brachyanthera. Kalopanaxsaponin I inhibits LPS (HY-D1056)-induced nitric oxide production in macrophages, with a IC50 of 80.25 μM.
For research use only. We do not sell to patients.
- CAS No.: 843668-13-3
- Formula: C69H112O34
- Molecular Weight:1485.61
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Kalopanaxsaponin I (6.25-100 μM; 24-25 h) inhibits LPS-induced nitric oxide production in RAW 264.7 macrophages with an IC50 of 80.25 μM, and exhibits no cytotoxicity at concentrations up to 100 μM[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
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Cell Line:RAW 264.7 macrophages
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Concentration:6.25, 12.5, 25, 50, 100 μM
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Incubation Time:24 h (NO inhibition assay); 25 h (MTT cytotoxicity assay)
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Result:Inhibited nitric oxide production by 5.47% at 6.25 μM, 11.76% at 12.5 μM, 25.83% at 25 μM, 34.12% at 50 μM, and 56.8% at 100 μM.
Reached an IC50 of 80.25 μM for nitric oxide inhibition.
Showed no cytotoxicity at all tested concentrations.
Chemical Information
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CAS No. 843668-13-3
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Molecular Weight 1485.61
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Formula C69H112O34
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SMILES
OC[C@H]([C@H]([C@@H]([C@H]1O)O)O[C@H]2[C@@H]([C@@H]([C@H]([C@@H](O2)C)O)O)O)O[C@H]1OC[C@H]([C@H]([C@@H]([C@H]3O)O)O)O[C@H]3OC([C@]45[C@](CC(C)(CC5)C)([H])C6=CC[C@@]([C@@]7([C@@]([C@@](C)([C@H](CC7)O[C@H]8[C@@H]([C@H]([C@H](CO8)O)O)O[C@H]9[C@@H]([C@@H]([C@H]([C@@H](O9)C)O)O[C@H]%10[C@@H]([C@H]([C@@H](CO%10)O[C@H]%11[C@@H]([C@H]([C@@H](CO%11)O)O)O)O)O)O)CO)([H])CC%12)C)([H])[C@]%12(C)[C@@]6(CC4)C)=O
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Synonyms
Septemloside I
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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Cell Cytotoxicity Assay
Cytotoxicity assays are usually based on the assessment of cell membrane damage, which can also be indirectly detected by measuring cell viability. Detection methods include MTT assay, CKK-8 assay, LDH assay and ATP assay, etc.
Purity & Documentation
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Kalopanaxsaponin I Related Classifications
HY-N17961 Related Classifications
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