6-NBDG
Based on 1 Customer Validation
6-NBDG is a fluorescent glucose analogue, it can be used for fluorescence imaging and monitoring glucose transport and uptake. 6-NBDG can be used as a fluorescent probe for detecting macrophage-rich atherosclerotic plaques.
For research use only. We do not sell to patients.
- Purity: 98.75%
- CAS No.: 108708-22-1
- Formula: C12H14N4O8
- Molecular Weight:342.26
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Storage:
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Cell Preparation
Culture cells to appropriate confluence on suitable imaging dishes or plates.
2. Glucose Starvation
Wash cells 2-3 times with warm PBS (without Mg2?/Ca2?) to remove existing glucose.
Incubate cells in glucose-free DMEM or KRH buffer for 30-60 minutes at 37°C to increase transporter activity.
3. 6-NBDG Incubation
Replace medium with glucose-free medium containing 50-150 μM 6-NBDG.
Incubate at 37°C for 15-60 minutes, protecting the samples from light (e.g., aluminum foil).
4. Washing and Measurement
Remove the 6-NBDG solution.
Wash cells 3 times with ice-cold PBS to remove non-specific fluorescence.
Measure immediately using a fluorescent plate reader, flow cytometer, or confocal microscope (Ex: 465-488 nm / Em: 530-542 nm).
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
542
488
Chemical Information
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CAS No. 108708-22-1
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Appearance Solid
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Molecular Weight 342.26
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Formula C12H14N4O8
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Color Orange to red
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SMILES
O=C[C@@H]([C@H]([C@@H]([C@@H](CNC1=CC=C([N+]([O-])=O)C2=NON=C21)O)O)O)O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
-20°C, protect from light, stored under nitrogen
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen)
Solvent & Solubility
DMSO : 10 mg/mL (29.22 mM; Need ultrasonic and warming; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (274 KB)
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SDS (394 KB)
- English - EN (394 KB)
- Français - FR (394 KB)
- Deutsch - DE (394 KB)
- Norwegian - NO (394 KB)
- Español - ES (394 KB)
- Swedish - SV (394 KB)
- Italian - IT (394 KB)
- Korean - KR (394 KB)
- Portuguese - PT (394 KB)
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Handling Instructions (2659 KB)
References
[1]. Hansen TV, et al. Glucose Absorption by the Bacillary Band of Trichuris muris. PLoS Negl Trop Dis. 2016 Sep 2;10(9):e0004971. [Content Brief]
[2]. Zaman RT, et al. Fiber-optic system for dual-modality imaging of glucose probes 18F-FDG and 6-NBDG in atherosclerotic plaques. PLoS One. 2014 Sep 18;9(9):e108108. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light, stored under nitrogen). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9218 mL | 14.6088 mL | 29.2176 mL | 73.0439 mL |
| 5 mM | 0.5844 mL | 2.9218 mL | 5.8435 mL | 14.6088 mL | |
| 10 mM | 0.2922 mL | 1.4609 mL | 2.9218 mL | 7.3044 mL | |
| 15 mM | 0.1948 mL | 0.9739 mL | 1.9478 mL | 4.8696 mL | |
| 20 mM | 0.1461 mL | 0.7304 mL | 1.4609 mL | 3.6522 mL | |
| 25 mM | 0.1169 mL | 0.5844 mL | 1.1687 mL | 2.9218 mL |
6-NBDG Related Classifications
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