3,4-Dimethylpyrazole phosphate
Based on 1 Customer Validation
3,4-Dimethylpyrazole phosphate (3,4-DMPP) is a nitrification inhibitor and microbial community regulator, whose activity is dose- and soil pH-dependent. 3,4-Dimethylpyrazole phosphate inhibits ammonia monooxygenase, ammonia-oxidizing bacteria, as well as ammonia-oxidizing archaea and nitrite-oxidizing bacteria. 3,4-Dimethylpyrazole phosphate alters the community composition of prokaryotes, fungi and ammonia-oxidizing bacteria. 3,4-Dimethylpyrazole phosphate reduces nitrate leaching, nitrous oxide emission and soil nitrate accumulation, and enhances nitrogen use efficiency and crop yield in agricultural production. 3,4-Dimethylpyrazole phosphate is applicable to agriculture-related research.
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- Purity : 98.0%
- CAS No.: 202842-98-6
- 화학식: C5H11N2O4P
- 분자량:194.13
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보관:
Store at room temperature 3 years.
In solvent -80°C, 2 years , -20°C, 1 year
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Biological Activity
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Chemical Information
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용액&용해도
- Protocol
- 순도&문서
- References
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Help & FAQs
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Biological Activity
제품 설명
In Vitro
3,4-Dimethylpyrazole phosphate exhibits dose-dependent persistence in acidic and alkaline loamy cambisols; its persistence is stronger in acidic soils than in alkaline soils; it inhibits nitrification in a dose- and soil pH-dependent manner; it selectively inhibits ammonia-oxidizing bacteria in alkaline loamy cambisols, whereas ammonia-oxidizing archaea in both acidic and alkaline loamy cambisols are only inhibited at high doses[1].
3,4-Dimethylpyrazole phosphate (1.54–450 mg/kg dry soil, treated for 14 and 35 days) at high doses selectively decreases the abundance of Nitrobacter in both acidic and alkaline loamy cambisols, while low doses boost the gene abundance of denitrifying bacteria and raise the quantities of total prokaryotes, fungi and Thaumarchaeota in alkaline loamy cambisols[1].
3,4-Dimethylpyrazole phosphate (applied at 2.32-23.2 mg DMPP per kg dry soil; on days 1, 7, and 14 post-treatment) inhibits nitrification and potential ammonia oxidation in grassland sandy loam supplemented with NH4+-N; it reduces the cell-specific nitrification activity of ammonia-oxidizing bacteria, ammonia-oxidizing archaea, or both[2].
3,4-Dimethylpyrazole phosphate (0.385-0.96 mg 100 g soil-1; 0-5 weeks) inhibits nitrification of swine manure-derived NH4+-N in Limburgerhof loamy sand in a concentration-dependent manner[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 202842-98-6
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Appearance Solid
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분자량 194.13
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화학식 C5H11N2O4P
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Color White to yellow
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SMILES
CC1=CNN=C1C.O=P(O)(O)O
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Synonyms
3,4-DMPP
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Store at room temperature 3 years
In solvent -80°C 2 years -20°C 1 year
용액&용해도
In Vitro:
DMSO : 100 mg/mL (515.12 mM; Need ultrasonic; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
In Vivo:
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 5 mg/mL (25.76 mM); Clear solution
This protocol yields a clear solution of ≥ 5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (50.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
In Vivo Dissolution Calculator
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL.
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Protocol
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Research Protocol for Microbiome Analysis
Microbiome analysis characterizes microbial communities in biological or environmental samples by measuring community composition, diversity, taxonomic structure, functional potential, and associations with host or environmental phenotypes. 16S rRNA gene amplicon sequencing is commonly used for bacterial and archaeal taxonomic profiling, while shotgun metagenomics provides higher taxonomic resolution and direct functional information, including microbial genes, pathways, viruses, fungi, and antimicrobial-resistance genes when sequencing depth and host-DNA contamination are adequately controlled. Microbiome results are strongly affected by sample collection, storage, DNA extraction, contamination, sequencing method, reference database, and bioinformatic pipeline; therefore, standardized protocols, negative controls, mock communities, and transparent analysis workflows are required. Unresolved issues include low-biomass contamination, compositional-data bias, inconsistent species-level c
순도&문서
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Data Sheet (273 KB)
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SDS (396 KB)
- English - EN (396 KB)
- Français - FR (396 KB)
- Deutsch - DE (396 KB)
- Norwegian - NO (396 KB)
- Español - ES (396 KB)
- Swedish - SV (396 KB)
- Italian - IT (396 KB)
- Korean - KR (396 KB)
- Portuguese - PT (396 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 2 years; -20°C, 1 year. When stored at -80°C, please use it within 2 years. When stored at -20°C, please use it within 1 year.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 5.1512 mL | 25.7559 mL | 51.5119 mL | 128.7797 mL |
| 5 mM | 1.0302 mL | 5.1512 mL | 10.3024 mL | 25.7559 mL | |
| 10 mM | 0.5151 mL | 2.5756 mL | 5.1512 mL | 12.8780 mL | |
| 15 mM | 0.3434 mL | 1.7171 mL | 3.4341 mL | 8.5853 mL | |
| 20 mM | 0.2576 mL | 1.2878 mL | 2.5756 mL | 6.4390 mL | |
| 25 mM | 0.2060 mL | 1.0302 mL | 2.0605 mL | 5.1512 mL | |
| 30 mM | 0.1717 mL | 0.8585 mL | 1.7171 mL | 4.2927 mL | |
| 40 mM | 0.1288 mL | 0.6439 mL | 1.2878 mL | 3.2195 mL | |
| 50 mM | 0.1030 mL | 0.5151 mL | 1.0302 mL | 2.5756 mL | |
| 60 mM | 0.0859 mL | 0.4293 mL | 0.8585 mL | 2.1463 mL | |
| 80 mM | 0.0644 mL | 0.3219 mL | 0.6439 mL | 1.6097 mL | |
| 100 mM | 0.0515 mL | 0.2576 mL | 0.5151 mL | 1.2878 mL |
3,4-Dimethylpyrazole phosphate Related Classifications
HY-W019334 Related Classifications
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