Bz-VGR-AMC
Bz-VGR-AMC is a fluorescent substrate for the trypsin-like activity of the 20S proteasome. Bz-VGR-AMC can be used to measure the trypsin-like (β2) activity of the 20S proteasome (Ex/Em = 360/460 nm).
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- CAS No.: 87779-49-5
- 화학식: C30H37N7O6
- 분자량:591.66
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- 순도&문서
- References
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Help & FAQs
Biological Activity
Guide (The following is our recommended protocol. This protocol is only a guide and should be modified according to your specific needs).
1. Take the tissue sample to be tested, dilute it with ddH2O, and mix it with the reaction buffer (250 mM HEPES, pH 7.5, 5 mM EDTA, 0.5% NP-40, 0.01% SDS, 5 mM ATP).
2. Add the fluorescent peptide Bz-VGR-AMC (10 μM) to the sample.
3. Incubate the reaction at 37°C for 30 minutes.
4. Monitor the fluorescence on a fluorescence microplate reader at a wavelength of Ex/Em = 360/460 nm.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 87779-49-5
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분자량 591.66
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화학식 C30H37N7O6
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Sequence
{Bz}-Val-Gly-Arg-{AMC}
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Sequence Shortening
{Bz}-VGR-{AMC}
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
References
[1]. Zhong YL, et al. Celastrol induce apoptosis of human multiple myeloma cells involving inhibition of proteasome activity. Eur J Pharmacol. 2019 Jun 15;853:184-192. [Content Brief]
[2]. Jarome TJ, et al. CaMKII, but not protein kinase A, regulates Rpt6 phosphorylation and proteasome activity during the formation of long-term memories. Front Behav Neurosci. 2013 Aug 30;7:115. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Bz-VGR-AMC Related Classifications
HY-137897 Related Classifications
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