HBT-Fl-BnB
HBT-Fl-BnB is a fluorescent probe for the ratiometric detection of ONOO- in vitro and in vivo. HBT-Fl-BnB consists of an HBT core with Fl groups at the ortho and para positions responding to the zwitterionic excited-state intramolecular proton-transfer (zwitterionic ESIPT) process and a boronic acid pinacol ester with dual roles that block the zwitterionic ESIPT and recognize ONOO-.
연구목적의 판매만을 진행합니다. 환자를 대상으로 한 판매는 하지 않습니다.
- 화학식: C75H88BNO3S
- 분자량:1094.38
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보관:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- 순도&문서
- References
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Help & FAQs
Biological Activity
제품 설명
In Vitro
Guide (Following is our recommended protocol. This protocol only provides a guideline, and should be modified according to your specific needs).
1. Raw264.7 cells are continued with different concentrations of HBT-Fl-BnB solution (0, 5, 10, 15, 20, 30, 50 μM) in the cell culture incubator.
2. After incubation for 24 h, dual channels for the confocal microscope are used to image the cells treated with HBT-Fl-BnB: the first channel (430ch) λem = 415?515 nm and the second channel (583ch) λex = 405 nm and λem = 550-650 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
Eight-week-old female C57BL/6 mice were selected. HBT-Fl-BnB (50 μM, 200 μL) is injected through the tail vein at 30 min before imaging. Then, these mice were imaged with the small animal in vivo imaging systems PerkinElmer IVIS Lumina XR and the Fluoroscopic Navigator 360I System. Dual channels for small animal in vivo imaging systems were used to image the mouse treated with HBT-Fl-BnB: the first channel (430ch) λem=415-515 nm and the second channel (583ch) λex=405 nm and λem=550-650 nm[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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분자량 1094.38
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화학식 C75H88BNO3S
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SMILES
CC(O1)(C)C(C)(C)OB1C(C=C2)=CC=C2OC3=C(C=C(C4=CC(C(C=CC=C5)=C5C6(CCCCCC)CCCCCC)=C6C=C4)C=C3C7=CC(C(C=CC=C8)=C8C9(CCCCCC)CCCCCC)=C9C=C7)C%10=NC%11=C(S%10)C=CC=C%11
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선적
Room temperature in continental US; may vary elsewhere.
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보관
Please store the product under the recommended conditions in the Certificate of Analysis.
순도&문서
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
HBT-Fl-BnB Related Classifications
HY-D2286 Related Classifications
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