Laxogenin
Laxogenin ((25R)-3β-Hydroxy-5α-spirostan-6-one; 6-Oxotigogenin) is an orally active myostatin (myostatin) inhibitor. Laxogenin promotes myogenesis and enhances the formation and maturation of myotubes. Laxogenin downregulates ROS. When added to the culture medium for cultured meat production, Laxogenin improves the texture, quality and nutritional value of cultured meat. Laxogenin reduces the average number of tumors in a two-stage mouse lung carcinogenesis model. Laxogenin can be used in research related to myogenesis and lung cancer.
For research use only. We do not sell to patients.
- CAS No.: 1177-71-5
- Formula: C27H42O4
- Molecular Weight:430.63
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Protocols
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
Description
In Vitro
Laxogenin (10 nM) promotes the differentiation of bovine bone marrow mesenchymal stem cells (MSCs) through the following mechanisms: increasing creatine kinase activity by 11%, upregulating the expression of myogenic markers, downregulating the expression of myostatin (MSTN), reducing reactive oxygen species (ROS) levels by 18%, and increasing myotube size[1].
Laxogenin (10 nM) promotes the differentiation of porcine mesenchymal stem cells (MSCs) through the following mechanisms: increasing creatine kinase activity by up to 18%, upregulating the expression of myogenic markers, downregulating MSTN expression, and reducing ROS levels by 7%[1].
Laxogenin (10 nM) promotes the differentiation of chicken bone marrow mesenchymal stem cells (MSCs) through the following mechanisms: increasing creatine kinase activity by 9-12%, upregulating the expression of myogenic markers, reducing the expression of MSTN, and decreasing reactive oxygen species (ROS) levels by 37%[1].
Laxogenin (10 nM) promotes the differentiation of C2C12 cells by increasing myotube size, upregulating MYH protein expression, enhancing creatine kinase activity by 9-19%, and upregulating the expression of myogenic markers over a 6-day differentiation period[1].
Laxogenin (10 nM) inhibits the MSTN signaling pathway in C2C12 cells, reduces ROS levels by approximately 8%, and upregulates the expression of antioxidant factors to promote cell differentiation[1].
Laxogenin (Compound 3) (100 μg/mL; pre-incubated for 1 h prior to the addition of 32Pi/TPA, followed by 4 h of incubation) inhibits TPA-induced 32Pi incorporation into phospholipids of HeLa cells by 82.1%[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only. Further protocols information, click here.
Chemical Information
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CAS No. 1177-71-5
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Molecular Weight 430.63
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Formula C27H42O4
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SMILES
C[C@@H]1[C@]2(O[C@@]3([C@]1([C@]4(C)[C@@](C3)([C@]5([C@](CC4)([C@]6(C)[C@@](C(=O)C5)(C[C@@H](O)CC6)[H])[H])[H])[H])[H])[H])CC[C@@H](C)CO2
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Synonyms
(25R)-3β-Hydroxy-5α-spirostan-6-one; 6-Oxotigogenin
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Structure Classification
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Initial Source
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Protocols
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ROS/oxidative-stress fluorescent staining
ROS/oxidative-stress fluorescent staining uses cell-permeant fluorogenic probes that become fluorescent after oxidation inside cells or tissues; commonly used examples include DCFH-DA/DCFDA for broad cellular oxidant detection, DHE for superoxide-related signal detection, MitoSOX for mitochondrial superoxide-related signal detection, and CellROX probes for oxidative-stress-associated fluorescence readouts. The assay detects probe oxidation rather than a single ROS species unless the probe and analysis method have been chemically validated for that species. DCFH-DA enters cells, is deacetylated by intracellular esterases to DCFH, and produces fluorescent DCF after oxidation, so the readout is used as an operational measure of total cellular oxidative stress rather than a species-specific ROS measurement. DHE and MitoSOX can report superoxide-related oxidation, but red fluorescence alone can include non-specific ethidium-like oxidation products; HPLC or optimized spectral approaches are
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C2C12 myoblast-to-myotube differentiation
C2C12 cells are mouse myoblast-lineage cells that proliferate in growth conditions and differentiate after mitogen reduction into elongated, multinucleated myotubes; the differentiation readout is generated by morphology, myogenic marker expression, and immunofluorescent detection of myosin heavy chain-positive myotubes with nuclear counterstaining.
Purity & Documentation
References
[1]. Lim JH, et al. The Effects of Laxogenin and 5-Alpha-hydroxy-laxogenin on Myotube Formation and Maturation During Cultured Meat Production. Int J Mol Sci. 2025;26(1):345. Published 2025 Jan 2. [Content Brief]
[2]. Baba M, et al. Saponins isolated from Allium chinense G. Don and antitumor-promoting activities of isoliquiritigenin and laxogenin from the same drug. Biol Pharm Bull. 2000 May;23(5):660-2. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Laxogenin Related Classifications
HY-N2457 Related Classifications
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