LysoTracker Blue DND-22
Based on 1 Customer Validation
LysoTracker blue DND-22 is a blue fluorescent dye (Ex/Em: 373/422 nm). LysoTracker blue DND-22 stains acidic regions in living cells. LysoTracker blue DND-22 is used in the researches of neurodegenerative diseases and leukemia.
For research use only. We do not sell to patients.
- Purity: 99.90%
- CAS No.: 215247-93-1
- Formula: C24H38Cl4N4
- Molecular Weight:524.40
-
Storage:
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
-
Biological Activity
-
Chemical Information
-
Solvent & Solubility
- Purity & Documentation
- References
-
Help & FAQs
Biological Activity
Guide (The following is our recommended protocol, which is provided as a guide only and should be modified to suit your specific needs).
Staining steps[2]
1) Under the conditions of 37°C and 5% CO2, U937 cells are cultured in IMDM medium containing 10% fetal bovine serum (HY-T1000), 100 units/mL Penicillin, and 50 μg/mL Streptomycin (HY-B1906).
2) Cells are treated with labeled oxLDL (18 μg/mL) or oxLDL-IC (24 μg/mL).
3) 50 nM LysoTracker Blue DND-22 is added to the cells 30 min before the end of the incubation with labeled oxLDL and oxLDL-IC.
4) After the incubation, cells are washed three times with serum-free IMDM to remove unbound dye.
5) Cells are suspended in 100 μL of IMDM, and then 10 μL aliquots are loaded into glass capillaries and sealed.
6) Cells are visualized using a confocal microscope with an excitation wavelength of 373 nm and an emission wavelength of 422 nm to observe lysosomal localization.
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
-
CAS No. 215247-93-1
-
Appearance Solid
-
Molecular Weight 524.40
-
Formula C24H38Cl4N4
-
Color Light yellow to yellow
-
SMILES
CN(CCNCC1=C2C=CC=CC2=C(CNCCN(C)C)C3=CC=CC=C31)C.Cl.Cl.Cl.Cl
-
Shipping
Room temperature in continental US; may vary elsewhere.
-
Storage
-20°C, sealed storage, away from moisture and light
* In solvent : -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light)
Solvent & Solubility
DMSO : 2 mg/mL (3.81 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
-
Data Sheet (275 KB)
-
SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Portuguese - PT (252 KB)
-
Handling Instructions (2659 KB)
References
[1]. Zeng J, et al. Biodegradable PLGA nanoparticles restore lysosomal acidity and protect neural PC-12 cells against mitochondrial toxicity. Ind Eng Chem Res. 2019 Aug 7;58(31):13910-13917. [Content Brief]
[2]. Al Gadban MM, et al. Differential trafficking of oxidized LDL and oxidized LDL immune complexes in macrophages: impact on oxidative stress. PLoS One. 2010 Sep 2;5(9):e12534. [Content Brief]
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (sealed storage, away from moisture and light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.9069 mL | 9.5347 mL | 19.0694 mL | 47.6735 mL |
Powered by Bioz