MAUra-DTB
MAUra-DTB is a 1-methyl-4-arylurazole (MAUra) chemical probe conjugated with desthiobiotin, which preferentially labels tyrosine residues exposed on protein surfaces under photocatalytic or laccase-catalyzed conditions (). The desthiobiotin moiety of MAUra-DTB supports affinity enrichment based on streptavidin or NeutrAvidin. MAUra-DTB enables proteome-wide labeling in cell lysates, and after enrichment of labeled peptides by NeutrAvidin, the labeling sites can be identified via nanoLC-MS/MS. MAUra-DTB can be used for photocatalytic proximity labeling, target protein identification and proteomic studies.
For research use only. We do not sell to patients.
- CAS No.: 2231747-83-2
- Formula: C25H38N6O5
- Molecular Weight:502.61
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Storage:
Please store the product under the recommended conditions in the Certificate of Analysis.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
MAUra-DTB (500 μM; 30 min) selectively labels nuclear proteins in proximity to HaloTag-H2B transiently expressed in HeLa cells, with localization confirmed via colocalization of streptavidin-Texas Red and nuclear Hoechst 33342 signals[2].
MAUra-DTB (500 μM; 10-60 min) preferentially labels highly surface-exposed tyrosine residues in A431 cell lysate proteomes, with 2017 single-modification sites identified after 10 minutes and over 3000 single-modification sites identified after 60 minutes, showing minimal tryptophan cross-reactivity[3].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2231747-83-2
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Molecular Weight 502.61
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Formula C25H38N6O5
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SMILES
O=C1N(C2=CC=C(C=C2)OCCCCCCNC(CCCCC[C@H]3NC(N[C@H]3C)=O)=O)C(NN1C)=O
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Please store the product under the recommended conditions in the Certificate of Analysis.
Purity & Documentation
References
[1]. Sato S, et al. 1-Methyl-4-aryl-urazole (MAUra) labels tyrosine in proximity to ruthenium photocatalysts. Chemical communications (Cambridge, England). 2018 Jun 05;54(46):5871-5874. [Content Brief]
[2]. Nakane K, et al. Switching of Photocatalytic Tyrosine/Histidine Labeling and Application to Photocatalytic Proximity Labeling. International journal of molecular sciences. 2022 Oct 02;23(19):11622. [Content Brief]
[3]. Nakane K, et al. Laccase-catalyzed tyrosine click reaction with 1-methyl-4-arylurazole: rapid labeling on protein surfaces. Chemical communications (Cambridge, England). 2024 Nov 28;60(96):14208-14211. [Content Brief]
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
MAUra-DTB Related Classifications
HY-186281 Related Classifications
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