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  5. Met-Enkephalin-Arg-Phe

Met-Enkephalin-Arg-Phe is a naturally occurring heptapeptide with analgesic activity.

For research use only. We do not sell to patients.

Custom Peptide Synthesis

Met-Enkephalin-Arg-Phe Chemical Structure

Met-Enkephalin-Arg-Phe Chemical Structure

CAS No. : 73024-95-0

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Met-Enkephalin-Arg-Phe Related Antibodies

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Description

Met-Enkephalin-Arg-Phe is a naturally occurring heptapeptide with analgesic activity[1].

In Vivo

Met-Enkephalin-Arg-Phe (10-100 nmol/mouse; i.c.v.; once) shows analgesic activity in mice[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model: Male Swiss Webster mice[1]
Dosage: 10-100 nmol/mouse
Administration: Intracerebralventricular injection, once
Result: Produced a dose-related inhibition of the tail-flick response. The effects peaked at 2-4 min and were dissipated by 10 min. The EC50 was 38.5 nmol/mouse.
Molecular Weight

877.02

Formula

C42H56N10O9S
CAS No.
Sequence

Tyr-Gly-Gly-Phe-Met-Arg-Phe
Sequence Shortening

YGGFMRF
Structure Classification
Initial Source
Shipping

Room temperature in continental US; may vary elsewhere.
Storage

Please store the product under the recommended conditions in the Certificate of Analysis.
Solvent & Solubility
In Vitro: 

H2O

Peptide Solubility and Storage Guidelines:

1.  Calculate the length of the peptide.

2.  Calculate the overall charge of the entire peptide according to the following table:

  Contents Assign value
Acidic amino acid Asp (D), Glu (E), and the C-terminal -COOH. -1
Basic amino acid Arg (R), Lys (K), His (H), and the N-terminal -NH2 +1
Neutral amino acid Gly (G), Ala (A), Leu (L), Ile (I), Val (V), Cys (C), Met (M), Thr (T), Ser (S), Phe (F), Tyr (Y), Trp (W), Pro (P), Asn (N), Gln (Q) 0

3.  Recommended solution:

Overall charge of peptide Details
Negative (<0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, add NH4OH (<50 μL).
3.  If the peptide still does not dissolve, add DMSO (50-100 μL) to solubilize the peptide.
Positive (>0) 1.  Try to dissolve the peptide in water first.
2.  If water fails, try dissolving the peptide in a 10%-30% acetic acid solution.
3.  If the peptide still does not dissolve, try dissolving the peptide in a small amount of DMSO.
Zero (=0) 1.  Try to dissolve the peptide in organic solvent (acetonitrile, methanol, etc.) first.
2.  For very hydrophobic peptides, try dissolving the peptide in a small amount of DMSO, and then dilute the solution with water to the desired concentration.
Purity & Documentation
References
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  • Molarity Calculator

  • Dilution Calculator

The molarity calculator equation

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Mass   Concentration   Volume   Molecular Weight *
= × ×

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2
Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

Met-Enkephalin-Arg-Phe73024-95-0OthersInhibitorinhibitorinhibit

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