Methoxy-X04 (solution) 

Methoxy-X04 (solution) is a fluorescent dye that crosses the blood-brain barrier and selectively binds to beta-pleated sheets found in dense core amyloid Aβ plaques. Methoxy-X04 retains in vitro binding affinity for amyloid b (Ab) fibrils (K_i= 26.8 nM). Methoxy-X04 is fluorescent and stains plaques, tangles, and cerebrovascular amyloid in postmortem sections of AD brain with good specificity^[1][2].
Solvent and concentration: DMSO: 5 mg/mL

Methoxy-X04 (5-10 mg/kg, i.p., once time) differentiates individual plaques in PS1/APP mice within 30 to 60 min^[2].

1. Methoxy-X04 Solution Injection
24 hr prior to perfusion, weigh the mice and give each mouse an intraperitoneal injection of methoxy-X04 at a dose of 10 mg/kg of body weight.
2. Transcardial Perfusion of the Injected Mice
3. Brain Sectioning Using a Vibratome
4. Section Screening for the Presence of Methoxy-X04-stained Plaques
Examine each section successively, to avoid drying out the sections, using the following procedure:
Add a droplet of PBS to a microscope slide with a disposable pipette, and place the section on the droplet.
Examine the section under a fluorescent microscope (excitation 340-380 nm) to identify regions containing Methoxy-X04 labeled Aβ plaques.

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

344.41

C₂₃H₂₀O₃

863918-78-9

COC1=CC(/C=C/C2=CC=C(O)C=C2)=CC=C1/C=C/C3=CC=C(O)C=C3

Room temperature in continental US; may vary elsewhere.

Please store the product under the recommended conditions in the Certificate of Analysis.

• [1]. Klunk WE, et al. Imaging Abeta plaques in living transgenic mice with multiphoton microscopy and methoxy-X04, a systemically administered Congo red derivative. J Neuropathol Exp Neurol. 2002 Sep;61(9):797-805.  [Content Brief]

  [2]. Bisht K, et al. Correlative Light and Electron Microscopy to Study Microglial Interactions with β-Amyloid Plaques. J Vis Exp. 2016 Jun 1;(112):54060.  [Content Brief]