Micrococcal nuclease
Based on 1 Customer Validation
Micrococcal nuclease is a secreted nuclease from Staphylococcus aureus. It digests single-stranded and double-stranded DNA (ssDNA and dsDNA) as well as RNA, cleaves oligonucleotide linkers with T-T sites, and cuts neutrophil extracellular traps and biofilm extracellular DNA into mononucleotides and dinucleotides. Micrococcal nuclease stimulates the formation of Staphylococcus aureus biofilms and mediates immune evasion. It triggers on-demand release of antibiotics from hydrogel coatings, prolongs the formation of neutrophil extracellular traps, and promotes the dissemination and survival of MRSA during infection. Micrococcal nuclease is applicable to research and characterization related to Staphylococcus aureus infection.
Nur für Forschungszwecke. Wir verkaufen nicht an Patienten.
- CAS. Nr.: 9013-53-0
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Speicherung:
Please store the product under the recommended conditions in the Certificate of Analysis.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biologische Aktivität
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Technical Parameters
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Chemical Information
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Lösungsmittel & Löslichkeit
- Protokoll
- Reinheit & Dokumentation
- Verweise
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Help & FAQs
Biologische Aktivität
Beschreibung
In Vitro
Micrococcal nuclease (24 h) cleaves unmodified oligonucleotide linkers (0PS) faster than phosphorothioate-modified linkers (6PS), thereby releasing ampicillin and resulting in larger bacterial growth inhibition zones on S. aureus Xen29 agar cultures[2].
Micrococcal nuclease (derived from *Staphylococcus aureus* Newman WT lux) increases the bacterial density of biofilms on hydrophilic surfaces to 0.5 bacteria/mm3[3].
Micrococcal nuclease (24 h) cleaves unmodified 0PS oligonucleotide linkers faster than phosphorothioate-modified 6PS linkers, resulting in a higher amount of ampicillin released from hydrogel coatings[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
In Vivo
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
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Animal Model:Balb/c OlaHsd (female, 4-5 weeks old, average weight 20 g, subcutaneous implantation of polyvinylidene fluoride mesh followed by *Staphylococcus aureus* inoculation)[3]
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Dosage:Endogenous production via inoculated S. aureus Newman WT lux (108 CFUs)
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Administration:subcutaneous injection at implant site; single dose; day 0
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Result:Showed significantly higher bioluminescence (bacterial load) from day 1 to day 7 compared to nuclease-deficient mutant group, with no significant 3- to 10-fold signal decrease.
Recovered significantly more CFUs from mesh-tissue samples at day 7 compared to nuclease-deficient mutant group.
Displayed significantly higher S. aureus levels, myeloperoxidase levels, and citrullinated histone levels at day 7, with biofilms detected at the mesh-tissue interface.
Detected bacterial biofilms at the mesh-tissue interface at 12-13 days, with myeloperoxidase and citrullinated histones colocalized with biofilm structures.
Detected no foreign body giant cells in samples at either 7 or 12-13 days.
Technical Parameters
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Source
E.coli
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pH Stability
7.0-10.0
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Activators
Ca2+
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Inhibitors
100 mM salt ions
Chemical Information
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CAS. Nr. 9013-53-0
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Appearance Liquid
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Color Colorless to light yellow
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SMILES
[Micrococcal nuclease]
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Versand
Room temperature in continental US; may vary elsewhere.
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Speicherung
Please store the product under the recommended conditions in the Certificate of Analysis.
Lösungsmittel & Löslichkeit
In Vitro:
H2O : ≥ 20 mg/mL
* "≥" means soluble, but saturation unknown.
Reinheit & Dokumentation
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Data Sheet (270 KB)
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SDS (558 KB)
- English - EN (558 KB)
- Français - FR (558 KB)
- Deutsch - DE (558 KB)
- Norwegian - NO (558 KB)
- Español - ES (558 KB)
- Swedish - SV (558 KB)
- Italian - IT (558 KB)
- Korean - KR (558 KB)
- Portuguese - PT (558 KB)
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Handling Instructions (2659 KB)
Verweise
Calculators
Konzentration (Stammlösung) × Volumen (Stammlösung) = Konzentration (Ziellösung) × Volumen (Ziellösung)
Micrococcal nuclease Related Classifications
HY-P2754 Related Classifications
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Keywords
- Micrococcal nuclease
- 9013-53-0
- Endonuclease
- Bacterial
- mouse peritonitis model
- Staphylococcus aureus
- MRSA
- neutrophil extracellular trap
- double-stranded DNA
- methicillin-resistant Staphylococcus aureus infections
- biofilm extracellular DNA
- oligonucleotide linkers
- periprosthetic joint infections
- Inhibitor
- inhibitor
- inhibit