MK-4721  (Synonyms: AGS-1C4D4; AGS-PSCA)

AGS-1C4D4 is a fully human IgG1κ monoclonal antibody targeting PSCA, with a K_d value of 0.2 nM. AGS-1C4D4 mediates antibody-dependent cellular cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) against PSCA-expressing cells. AGS-1C4D4 can be used for the research of pancreatic cancer^[1].

Human IgG1 kappa

Human IgG1 kappa, Isotype Control

Human

PSCA

AGS-1C4D4 displays potent ADCC activity against PSCA-expressing HPAC and Panc0203 pancreatic cell lines, with activity greatly reduced by deglycosylation, and is more potent than the hybridoma-derived AGS-PSCA antibody in this assay^[1].
AGS-1C4D4 exhibits CDC activity against PSCA-expressing recombinant B300.19 cells, with activity greatly reduced by deglycosylation, and is equally effective as the hybridoma-derived AGS-PSCA antibody in this assay^[1].
AGS-1C4D4 binds to PSCA with a K_d of 0.2 nM, displaying similar binding affinity to the hybridoma-derived AGS-PSCA antibody^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

AGS-1C4D4 significantly inhibits established pancreatic tumor growth and metastasis in orthotopic HPAC xenograft models when combined with Gemcitabine (HY-17026), and also significantly inhibits pancreatic tumor formation, local invasion, and distant metastasis in the same orthotopic xenograft model^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

8000  [NCBI]

O43653

ELISA, FACS, Functional assay

Unconjugated

The product can be reconstituted/diluted with sterile PBS or saline.

143.25 kDa

Liquid

Colorless to light yellow

[MK-4721]

Shipping with dry ice.

Please refer to the lot-specific COA for specific buffer information.

Please store the product under the recommended conditions in the Certificate of Analysis.

• 
  Flow Cytometry analysis of HepG2 cells with MK-4721 (HY-P991229, red) . Cells were fixed with 4% paraformaldehyde and permeabilised with 90% methanol. Then cells were stained with the primary antibody at 1/200 dilution for an hour at 4℃. Goat Anti-Human IgG H&L (AF488) (HY-P83776) was used as the secondary antibody at 1/1,000 dilution for 30 minutes at 4℃. Human IgG1 kappa(HY-P99001, blue) was used as the isotype control, cells without incubation with primary antibody were used as the unlabeled control (black).

• [1]. Tony Navas, et al. Abstract 4566: AGS-1C4D4: A fully human anti-PSCA antibody inhibits tumor formation and metastasis in orthotopic models of pancreatic cancer. Cancer Res (2011) 71 (8_Supplement): 4566.