MrgprX2 antagonist-8
Based on 1 Customer Validation
MrgprX2 antagonist-8 (example 5b) is a MrgprX2 antagonist. MrgprX2 antagonist-8 can be used for the research of inflammatory disorders.
For research use only. We do not sell to patients.
- Purity: 97.00%
- CAS No.: 2768216-96-0
- Formula: C24H24ClF3N4O3S
- Molecular Weight:540.99
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Storage:
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
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Biological Activity
IC50 & target: MrgprX2[1]
MrgprX2 antagonist-8 has an activity of 100-500 nM in the hexokinase assay using LAD2 mast cells[1].
MrgprX2 antagonist-8 has an activity of less than 100 nM in CHO cells[1].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 2768216-96-0
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Appearance Solid
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Molecular Weight 540.99
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Formula C24H24ClF3N4O3S
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Color White to off-white
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SMILES
O=C(N[C@H]1CC[C@@H](NC2=CC(C(F)(F)F)=NC3=CC=C(Cl)C=C23)CC1)C4=CC=CC(NS(=O)(C)=O)=C4
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
4°C, protect from light
* In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
Solvent & Solubility
DMSO : ≥ 250 mg/mL (462.12 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
* "≥" means soluble, but saturation unknown.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Select the appropriate dissolution method based on your experimental animal and administration route.
- For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
- To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for In Vivo experiments, it is recommended to prepare freshly and use it on the same day.
- The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.
Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.08 mg/mL (3.84 mM); Clear solution
This protocol yields a clear solution of ≥ 2.08 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (20.8 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Please enter the basic information of animal experiments:
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Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.
Please enter your animal formula composition:
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%DMSO +
Recommended: Keep the proportion of DMSO in working solution below 2% if your animal is weak.
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%+
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+%Tween-80 + +
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%Saline +
The co-solvents required include: DMSO, . All of co-solvents are available by MedChemExpress (MCE). , Tween 80. All of co-solvents are available by MedChemExpress (MCE).
Working solution concentration: 0.22 mg/mL
Method for preparing stock solution: mg drug dissolved in μL DMSO. Stock solution concentration: mg/mL. * In solvent : -80°C, 6 months; -20°C, 1 month (protect from light)
1. Take μL DMSO stock solution;
2. Add μL .
μL , mix evenly;
3. Then add μL Tween 80, mix evenly;
4. Then add μL
Please ensure that the stock solution in the first step is dissolved to a clear state, and add co-solvents in sequence. You can use ultrasonic heating (ultrasonic cleaner, recommended frequency 20-40 kHz), vortexing, etc. to assist dissolution.
Purity & Documentation
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Data Sheet (271 KB)
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SDS (393 KB)
- English - EN (393 KB)
- Français - FR (393 KB)
- Deutsch - DE (393 KB)
- Norwegian - NO (393 KB)
- Español - ES (393 KB)
- Swedish - SV (393 KB)
- Italian - IT (393 KB)
- Portuguese - PT (393 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month (protect from light). When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 1.8485 mL | 9.2423 mL | 18.4846 mL | 46.2116 mL |
| 5 mM | 0.3697 mL | 1.8485 mL | 3.6969 mL | 9.2423 mL | |
| 10 mM | 0.1848 mL | 0.9242 mL | 1.8485 mL | 4.6212 mL | |
| 15 mM | 0.1232 mL | 0.6162 mL | 1.2323 mL | 3.0808 mL | |
| 20 mM | 0.0924 mL | 0.4621 mL | 0.9242 mL | 2.3106 mL | |
| 25 mM | 0.0739 mL | 0.3697 mL | 0.7394 mL | 1.8485 mL | |
| 30 mM | 0.0616 mL | 0.3081 mL | 0.6162 mL | 1.5404 mL | |
| 40 mM | 0.0462 mL | 0.2311 mL | 0.4621 mL | 1.1553 mL | |
| 50 mM | 0.0370 mL | 0.1848 mL | 0.3697 mL | 0.9242 mL | |
| 60 mM | 0.0308 mL | 0.1540 mL | 0.3081 mL | 0.7702 mL | |
| 80 mM | 0.0231 mL | 0.1155 mL | 0.2311 mL | 0.5776 mL | |
| 100 mM | 0.0185 mL | 0.0924 mL | 0.1848 mL | 0.4621 mL |
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