MTT Formazan
Based on 1 Customer Validation
MTT Formazan is a purple water-insoluble product formed by the reduction of yellow soluble MTT by succinate dehydrogenase in the mitochondria of viable cells. The amount of MTT Formazan produced is proportional to cellular metabolic activity, and it can serve as an indicator for evaluating cell viability and proliferation. MTT Formazan exhibits solvent-dependent solubility properties, and its absorbance value can be measured by spectrophotometry to reflect cellular status. MTT Formazan also supports the assessment of cell viability in bacterial MTT assays. MTT Formazan can be used in photoacoustic microscopy to enhance the imaging effect of viable cells, especially in applications in the fields of three-dimensional cell culture and tissue engineering.
For research use only. We do not sell to patients.
- Purity: 99.67%
- CAS No.: 57360-69-7
- Formula: C18H17N5S
- Molecular Weight:335.43
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Storage:Powder -20°C, 3 years , 4°C, 2 years ; In solvent -80°C, 6 months , -20°C, 1 month
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Chemical Information
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Solvent & Solubility
- Purity & Documentation
- References
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Help & FAQs
Biological Activity
MTT Formazan (1×109 cells/mL; 1-24 h) is generated by co-incubation of Staphylococcus aureus with MTT (HY-15924). When dissolved in DMF buffered with 5% SDS or DMSO buffered with 5% SDS, MTT Formazan exhibits a stable linear correlation with cell density for at least 24 h within the range up to 1×109 cells/mL, with high detection reproducibility[1].
MTT Formazan is produced by co-incubating NIH/3T3 fibroblasts with MTT, and its absorbance value increases linearly with cell density within the range of 3.125×103 to 1.156×105 cells/cm2; when dissolved with DMSO, the peak absorbance reaches 1.31 at a cell density of 1.156×105 cells/cm2[2].
MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.
Chemical Information
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CAS No. 57360-69-7
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Appearance Solid
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Molecular Weight 335.43
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Formula C18H17N5S
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Color Brown to black
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SMILES
CC1=C(C)N=C(N/N=C(C2=CC=CC=C2)/N=N/C3=CC=CC=C3)S1
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Shipping
Room temperature in continental US; may vary elsewhere.
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Storage
Powder -20°C 3 years 4°C 2 years In solvent -80°C 6 months -20°C 1 month
Solvent & Solubility
DMSO : 4 mg/mL (11.92 mM; ultrasonic and warming and heat to 60°C; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Purity & Documentation
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Data Sheet (273 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
References
Complete Stock Solution Preparation Table
Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.
| Optional Solvent | Concentration Solvent Mass | 1 mg | 5 mg | 10 mg | 25 mg |
|---|---|---|---|---|---|
| DMSO | 1 mM | 2.9812 mL | 14.9062 mL | 29.8125 mL | 74.5312 mL |
| 5 mM | 0.5962 mL | 2.9812 mL | 5.9625 mL | 14.9062 mL | |
| 10 mM | 0.2981 mL | 1.4906 mL | 2.9812 mL | 7.4531 mL |
MTT Formazan Related Classifications
HY-137006 Related Classifications
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