N-Butyl-N-(4-hydroxybutyl)nitrosamine (Synonyms: BBN)

Name: N-Butyl-N-(4-hydroxybutyl)nitrosamine
Brand: MedChemExpress
SKU: HY-W755252
Rating: 4.5 (1 reviews)

Cat. No.: HY-W755252 Pureza: 99.70%: Ficha de datos
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N-Butyl-N-(4-hydroxybutyl)nitrosamine is a potent mutagen that can cause high-level of mutagenesis specifically in the epithelial cells (urothelial) of the urinary bladder. N-Butyl-N-(4-hydroxybutyl)nitrosamine is used to induce bladder cancer in rodents.

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N-Butyl-N-(4-hydroxybutyl)nitrosamine Estructura química

No. CAS : 3817-11-6

Tamaño	Stock	Cantidad
Liquid + Solvent (Highly Recommended)
10 mM * 1 mL in DMSO ready for reconstitution	En stock	Estimated Time of Arrival: December 31
Solution
10 mM * 1 mL in DMSO	En stock	Estimated Time of Arrival: December 31
Liquid
100 mg	En stock	Estimated Time of Arrival: December 31
500 mg	En stock	Estimated Time of Arrival: December 31
1 g	En stock	Estimated Time of Arrival: December 31
5 g	Obtener un presupuesto
10 g	Obtener un presupuesto

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Based on 1 publication(s) in Google Scholar

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Descripciòn

In Vivo

Note:
Please do not refer to only one article to determine the experimental conditions. It is recommended to determine the optimal experimental conditions (animal strain, age, dosage, frequency and cycle, detection time and indicators, etc.) through preliminary experiments before the formal experiment.

N-Butyl-N-(4-hydroxybutyl)nitrosamine (BBN; 0.05% solution in drinking water; for 2 weeks) is a powerful mutagen in urothelial tissue in mice and rats^[1].

MedChemExpress (MCE) has not independently confirmed the accuracy of these methods. They are for reference only.

Animal Model:	Male and female lacI C57BL/6 homozygous mice and male lacI Fisher 344 rats^[1]
Dosage:	0.05% solution in drinking water
Administration:	Oral; for 2 weeks
Result:	The mutantfractions were extremely high in the bladder tissues.

Ensayo clínico

Peso molecular

174.24

Fòrmula

C₈H₁₈N₂O₂

No. CAS

3817-11-6

Appearance

Liquid (Density: 1.022±0.14 g/cm³)

Color

Colorless to light yellow

SMILES

CCCCN(N=O)CCCCO

Envío

Room temperature in continental US; may vary elsewhere.

Almacenamiento

Pure form	-20°C	3 years
	4°C	2 years
In solvent	-80°C	6 months
	-20°C	1 month

Solvente y solubilidad

In Vitro:

DMSO : ≥ 100 mg/mL (573.92 mM; Hygroscopic DMSO has a significant impact on the solubility of product, please use newly opened DMSO)

H₂O : 20 mg/mL (114.78 mM; Need ultrasonic)

*"≥" means soluble, but saturation unknown.

Preparing
Stock Solutions

Concentration Solvent Mass	1 mg	5 mg	10 mg

1 mM	5.7392 mL	28.6961 mL	57.3921 mL
5 mM	1.1478 mL	5.7392 mL	11.4784 mL
10 mM	0.5739 mL	2.8696 mL	5.7392 mL

View the Complete Stock Solution Preparation Table

* Please refer to the solubility information to select the appropriate solvent. Once prepared, please aliquot and store the solution to prevent product inactivation from repeated freeze-thaw cycles.
Storage method and period of stock solution: -80°C, 6 months; -20°C, 1 month. When stored at -80°C, please use it within 6 months. When stored at -20°C, please use it within 1 month.

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

Calculadora de molaridad
Calculadora de dilución

Mass (g) = Concentration (mol/L) × Volume (L) × Molecular Weight (g/mol)

Concentration _(start) × Volume _(start) = Concentration _(final) × Volume _(final)

This equation is commonly abbreviated as: C₁V₁ = C₂V₂

Concentration _(start)

Volume _(start)

Concentration _(final)

Volume _(final)

In Vivo:

Select the appropriate dissolution method based on your experimental animal and administration route.

For the following dissolution methods, please ensure to first prepare a clear stock solution using an In Vitro approach and then sequentially add co-solvents:
To ensure reliable experimental results, the clarified stock solution can be appropriately stored based on storage conditions. As for the working solution for in vivo experiments, it is recommended to prepare freshly and use it on the same day.
The percentages shown for the solvents indicate their volumetric ratio in the final prepared solution. If precipitation or phase separation occurs during preparation, heat and/or sonication can be used to aid dissolution.

Protocol 1

Add each solvent one by one: 10% DMSO 40% PEG300 5% Tween-80 45% Saline
Solubility: ≥ 2.5 mg/mL (14.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 400 μL PEG300, and mix evenly; then add 50 μL Tween-80 and mix evenly; then add 450 μL Saline to adjust the volume to 1 mL.
Preparation of Saline: Dissolve 0.9 g sodium chloride in ddH₂O and dilute to 100 mL to obtain a clear Saline solution.
Protocol 2

Add each solvent one by one: 10% DMSO 90% (20% SBE-β-CD in Saline)
Solubility: ≥ 2.5 mg/mL (14.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown).
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL 20% SBE-β-CD in Saline, and mix evenly.
Preparation of 20% SBE-β-CD in Saline (4°C, storage for one week): 2 g SBE-β-CD powder is dissolved in 10 mL Saline, completely dissolve until clear.
Protocol 3

Add each solvent one by one: 10% DMSO 90% Corn Oil
Solubility: ≥ 2.5 mg/mL (14.35 mM); Clear solution

This protocol yields a clear solution of ≥ 2.5 mg/mL (saturation unknown). If the continuous dosing period exceeds half a month, please choose this protocol carefully.
Taking 1 mL working solution as an example, add 100 μL DMSO stock solution (25.0 mg/mL) to 900 μL Corn oil, and mix evenly.

In Vivo Dissolution Calculator

Please enter the basic information of animal experiments:

Dosage

mg/kg

Animal weight
(per animal)

Dosing volume
(per animal)

μL

Number of animals

Recommended: Prepare an additional quantity of animals to account for potential losses during experiments.

Calculation results:

Working solution concentration: mg/mL

This product has good water solubility, please refer to the measured solubility data in water/PBS/Saline for details.

The concentration of the stock solution you require exceeds the measured solubility. The following solution is for reference only.If necessary, please contact MedChemExpress (MCE).

Pureza y Documentación

Purity: 99.70%

Select Batch:

Ficha de datos (269 KB) SDS (557 KB)

COA (241 KB) HNMR (250 KB) RP-HPLC (231 KB) LCMS (325 KB)

Instrucciones de manejo (2659 KB)

Referencias

[1]. Zhiming He, et al. Tissue-specific mutagenesis by N-butyl-N-(4-hydroxybutyl)nitrosamine as the basis for urothelial carcinogenesis. Mutat Res. 2012 Feb 18;742(1-2):92-5. [Content Brief]

Complete Stock Solution Preparation Table

Optional Solvent	Concentration Solvent Mass	1 mg	5 mg	10 mg	25 mg

H₂O / DMSO	1 mM	5.7392 mL	28.6961 mL	57.3921 mL	143.4803 mL
	5 mM	1.1478 mL	5.7392 mL	11.4784 mL	28.6961 mL
	10 mM	0.5739 mL	2.8696 mL	5.7392 mL	14.3480 mL
	15 mM	0.3826 mL	1.9131 mL	3.8261 mL	9.5654 mL
	20 mM	0.2870 mL	1.4348 mL	2.8696 mL	7.1740 mL
	25 mM	0.2296 mL	1.1478 mL	2.2957 mL	5.7392 mL
	30 mM	0.1913 mL	0.9565 mL	1.9131 mL	4.7827 mL
	40 mM	0.1435 mL	0.7174 mL	1.4348 mL	3.5870 mL
	50 mM	0.1148 mL	0.5739 mL	1.1478 mL	2.8696 mL
	60 mM	0.0957 mL	0.4783 mL	0.9565 mL	2.3913 mL
	80 mM	0.0717 mL	0.3587 mL	0.7174 mL	1.7935 mL
	100 mM	0.0574 mL	0.2870 mL	0.5739 mL	1.4348 mL

* Note: If you choose water as the stock solution, please dilute it to the working solution, then filter and sterilize it with a 0.22 μm filter before use.

N-Butyl-N-(4-hydroxybutyl)nitrosamine Related Classifications

Help & FAQs

Do most proteins show cross-species activity?
Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

Keywords:

N-Butyl-N-(4-hydroxybutyl)nitrosamine3817-11-6BBNDNA Alkylator/Crosslinkerbladder cancermutagenInhibitorinhibitorinhibit

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N-Butyl-N-(4-hydroxybutyl)nitrosamine (Synonyms: BBN)

Revisión del cliente

N-Butyl-N-(4-hydroxybutyl)nitrosamine Related Antibodies

Recomendaciones destacadas

•Related Screening Libraries:

•Related Small Molecules:

•Related Proteins:

Actividad biológica

Pureza y Documentación

Referencias

Revisión del cliente

Calculadora de molaridad

Calculadora de dilución

Complete Stock Solution Preparation Table

N-Butyl-N-(4-hydroxybutyl)nitrosamine Related Classifications

Keywords:

Productos vistos recientemente:

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SAFETY DATA SHEET (SDS)

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